1. [Effect of co-expression of nicotinic acid phosphoribosyl transferase and pyruvate carboxylase on succinic acid production in Escherichia coli BA002].
- Author
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Cao W, Gou D, Liang L, Liu R, Chen K, Ma J, and Jiang M
- Subjects
- Anaerobiosis, Escherichia coli enzymology, Fermentation, Genetic Engineering, Glucose metabolism, Industrial Microbiology, Lactococcus lactis enzymology, NAD metabolism, Pentosyltransferases genetics, Pyruvate Carboxylase genetics, Escherichia coli genetics, Escherichia coli metabolism, Pentosyltransferases biosynthesis, Pyruvate Carboxylase biosynthesis, Succinic Acid metabolism
- Abstract
Escherichia coli BA002, in which the ldhA and pflB genes are deleted, cannot utilize glucose anaerobically due to the inability to regenerate NAD+. To restore glucose utilization, overexpression of nicotinic acid phosphoribosyltransferase (NAPRTase) encoded by the pncB gene, a rate-limiting enzyme of NAD(H) synthesis pathway, resulted in a significant increase in cell mass and succinate production under anaerobic conditions. However, a high concentration of pyruvate was accumulated. Thus, co-expression of NAPRTase and the heterologous pyruvate carboxylase (PYC) of Lactococcus lactis subsp. cremoris NZ9000 in recombinant E. coli BA016 was investigated. Results in 3 L fermentor showed that OD600 is 4.64 and BA016 consumed 35.00 g/L glucose and produced 25.09 g/L succinate after 112 h under anaerobic conditions. Overexpression of pncB and pyc in BA016, the accumulation of pyruvic acid was further decreased, and the formation of succinic acid was further increased.
- Published
- 2013