1. EBNA-2-De\eted Epstein-Barr Virus from P3HR-1 Can Infect Rabbits with Lower Efficiency than Prototype Epstein-Barr Virus from B95-8.
- Author
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Sano, Hitoshi, Nagata, Keiko, Kato, Kaoru, Kanai, Kyousuke, Yamamoto, Kiyoshige, Okuno, Keisuke, Kuwamoto, Satoshi, Higaki-Mori, Hiromi, Sugihara, Hirotsugu, Kato, Masako, Murakami, Ichiro, Kanzaki, Susumu, and Hayashi, Kazuhiko
- Subjects
EPSTEIN-Barr virus ,LABORATORY rabbits ,PROTOTYPES ,VIRAL genetics ,VIRUS diseases ,GENE expression in viruses - Abstract
Objectives: To clarify characteristics on rabbit in vivo infec-tion with type 2 EBV nuclear antigen (EBNA-2)-de\eXed Ep-stein-Barr virus (P3HR-1-EBV) and compare infectious effica-cy of P3HR-1-EBV with previously reported prototype type 1 EBV from B95-8. Methods: Twelve Japanese White rabbits were inoculated with P3HR-1-EBV via intranasal or intrave-nous routes and autopsied on day 70-84. Results: In only 2 of 12 P3HR-1-EBV-inoculated rabbits, EBV-DNA was detected in peripheral blood mononuclear cells (PBMCs). ßomHI M rightward reading frame (BMRF)-l, EBNA-1 and ßamHI Z left-ward reading frame (BZLF)-1-vnRN/k were intermittently ex-pressed in PBMCs. In 1 infected rabbit with continuous de-tection of EBV-DNA in PBMCs, many EBER-1-positive lympho-cytes were observed in germinal centers and/or marginal zones in some follicles of the appendix, and for the first time a lymphocyte with EBER-1 expression infiltrating in the squa-mous cell layer of the tonsils was found. The other rabbit with a transient detection of EBV-DNA in PBMCs had no EBER-1-positive lymphocytes in the tissues examined. Few EBER-1-positive lymphocytes were detected in some rabbits with-out detection of EBV-DNA in PBMCs. Conclusions: P3HR-1-EBV showed less efficient infection in rabbits than EBV from the B95-8 cell line. However, a P3HR-1-EBV-inoculated animal model is meaningful because this is the first study of EBNA-2 function on in vivo EBV infection and it demonstrated the in vivo infectivity with lytic-type infection by EBNA-2-deleted EBV. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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