1. The value of anti-desmoglein enzyme-linked immunosorbent assay in the immunological follow-up of pemphigus.
- Author
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Bellon N, André C, Sbidian E, Ortonne N, Wolkenstein P, Chosidow O, and Ingen-Housz-Oro S
- Subjects
- Adult, Aged, Databases, Factual, Disease Progression, Enzyme-Linked Immunosorbent Assay methods, Female, Fluorescent Antibody Technique, Direct methods, Fluorescent Antibody Technique, Indirect methods, Follow-Up Studies, France, Humans, Male, Middle Aged, Retrospective Studies, Sensitivity and Specificity, Severity of Illness Index, Statistics, Nonparametric, Young Adult, Desmoglein 1 analysis, Desmoglein 1 blood, Desmoglein 3 blood, Monitoring, Physiologic methods, Pemphigus blood, Pemphigus physiopathology
- Abstract
Background: The value of anti-desmoglein 1 and 3 (Dsg1, Dsg3) enzyme-linked immunosorbent assay (ELISA) is controversial in the follow-up of pemphigus., Objective: To evaluate anti-desmoglein ELISA (Dsg ELISA) in the follow-up of pemphigus and compare ELISA with direct and indirect immunofluorescence in complete remission (CR)., Methods: We performed a retrospective monocenter study of patients with pemphigus and consecutive sera samples collected at baseline (M0), 12 months (M12) and 24 or 36 months after M0 (M24/36). Tests were compared in CR and in active disease. Direct immunofluorescence and circulating autoantibodies were compared for patients with stable CR., Results: We included 36 patients. At M12, ELISA values did not differ between CR and active disease. At M24/36, Dsg3 but not Dsg1 ELISA values were lower in CR (p = 0.07). For 5/8 patients with stable CR, direct immunofluorescence and ELISA findings remained positive., Conclusion: In routine practice, Dsg ELISA seems to be of little interest for immunological follow-up of pemphigus.
- Published
- 2014
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