1. Role of Wnt signaling on proliferation of menstrual blood derived stem cells
- Author
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Iraj Nikokar, Amir-Hassan Zarnani, S Saghari, Manijeh Khanmohammadi, and Somaieh Kazemnejad
- Subjects
Pathology ,medicine.medical_specialty ,proliferation ,lcsh:Medicine ,ß-catenin ,Immunofluorescence ,Biochemistry ,Immunophenotyping ,medicine ,MTT assay ,Molecular Biology ,lcsh:R5-920 ,Menstrual blood derived stem cells ,medicine.diagnostic_test ,Cell growth ,business.industry ,lcsh:R ,Mesenchymal stem cell ,Wnt signaling pathway ,Cell Biology ,Embryonic stem cell ,Cell biology ,Lithium chloride ,Stem cell ,lcsh:Medicine (General) ,business ,Biotechnology ,Research Article - Abstract
Aim: Menstrual blood derived stem cells (MenSCs) are unique stem cells that have been isolated and identified recently. The special traits of MenSCs can be related to the cell signaling pathways. In this study, in order to find out the role of Wnt signaling on MenSCs proliferation, we evaluated ß-catenin expression as a key participant in Wnt signaling pathway in response to Lithium chloride (LiCl). Methods: MenSCs were isolated from healthy women by combining gradient density centrifugation with plastic adherence. After characterization of the isolated cells, cell proliferation of MenSCs in presence of 10-15 mM LiCl was evaluated by MTT assay. ß-catenin expression of the treated cells was examined using immunofluorescence technique. Results: Flow cytometric analysis revealed that both mesenchymal and embryonic stem cell markers are expressed on menstrual blood stem cells. MTT value decreased depending on the LiCl concentration. The proliferation of MenSCs cultivated in culture media containing 15mM LiCl was approximately two fold less than those grown without LiCl (p
- Published
- 2013