Objective To understand the microbial contamination status of main living and drinking water in Pukou Dis-trict of Nanking City to provide reference for improving the quality of living and drinking water. Methods According to the GB/T5750-2006 Living and Drinking Water Standard Examination Method, the 3 kinds of living water of urban centralized water sup-ply and rural centralized water supply tap water, secondary water supply and direct drinking water were detected, including the total number of bacterial colonies, total coliform group, thermotolerant coliform group, etc. Results Total by 526 living and drinking water samples were detected during 2013-2015, 492 samples were qualified with the qualified rate of 93.5%. The quali-fied rates of 3-kind living and drinking water in 2013 were 87.2%,95.7%and 50.0% respectively, which in 2014 were 90.6%, 96.7% and 75.0% respectively, which in 2015 were 92.1%,97.4% and 42.9% respectively; the qualified rate of living and drink ing water in the same year during 2013-2015 had statistically significant difference among 3 kinds of water (χ2=9.64, 8.57, 28.24, P<0.05). The qualified rate of the same kind of living and drinking water in tap water, secondary water supply and direct drinking water had no statistical difference among 2013-2015(χ2=0.56,0.49,1.67, P>0.05);the qualified rates of tap water were 93.7%for urban tap water and 82.6%for rural tap water, the difference was statistically significant(χ2=4.25, P<0.05). The qualified rates of total number of bacterial colonies in tap water, secondary water supply and direct drinking water were 90.1%,96.5%and 58.8%respectively, the difference was statistically significant(χ2=41.91, P<0.01). Conclusion The living and drinking water microbial indicators of urban centralized water supply is higher than that of rural centralized water supply, the qualified rate of bacterial colony total number of 3-kind living and drinking water is lowest, especially direct drinking water. The relevant depart-ments should take measures to improve the quality of living and drinking water for ensuring the drinking water safety of masses. [ABSTRACT FROM AUTHOR]