1. Zinc binding to human lactogenic hormones and the human prolactin receptor.
- Author
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Voorhees JL, Rao GV, Gordon TJ, and Brooks CL
- Subjects
- Binding Sites, Fluorescence Resonance Energy Transfer, Human Growth Hormone metabolism, Humans, Placental Lactogen metabolism, Prolactin metabolism, Protein Conformation, Pituitary Hormones metabolism, Receptors, Prolactin metabolism, Zinc metabolism
- Abstract
Zinc half sites are present in all human lactogenic hormones: human prolactin (hPRL), growth hormone (hGH), placental lactogens (hPL) and the hPRL receptor (hPRLr). The influence of divalent zinc (Zn(2+)) as measured by intrinsic fluorescence or FRET in each of these hormones is unique and is affected by the presence of varying stoichiometries of hPRLr. These data show that both Zn(2+) and hPRLr binding influence hPRL conformers in an interdependent fashion. Although each of these three lactogenic hormones bind hPRLr and induce a biological response that is sensitive to the presence of increasing concentrations of Zn(2+), each hormone is unique in the mechanistic details of this process., (Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)
- Published
- 2011
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