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Your search keyword '"Sanzari E"' showing total 2 results
Start Over Author "Sanzari E" Publisher john wiley & sons ltd
2 results on '"Sanzari E"'

1. Characterization of the human STAT5A and STAT5B promoters: evidence of a positive and negative mechanism of transcriptional regulation.

Author

Crispi S, Sanzari E, Monfregola J, De Felice N, Fimiani G, Ambrosio R, D'Urso M, and Ursini MV

Subjects
Animals, Binding Sites, Cell Line, Tumor, DNA-Binding Proteins metabolism, Drosophila melanogaster genetics, Drosophila melanogaster metabolism, Humans, Mice, STAT5 Transcription Factor, Sequence Analysis, DNA, Trans-Activators metabolism, Tumor Suppressor Proteins, DNA-Binding Proteins genetics, Gene Expression Regulation, Milk Proteins, Promoter Regions, Genetic, Trans-Activators genetics, Transcription, Genetic
Abstract

We recently published the genomic characterization of the STAT5A and STAT5B paralogous genes that are located head to head in the 17q21 chromosome and share large regions of sequence identity. We here demonstrate by transient in vitro transfection that STAT5A and STAT5B promoters are able to direct comparable levels of transcription. The expression of basal promoters is enhanced after Sp1 up-regulation in HeLa and SL2 cells while DNA methylation associated to the recruitment of MeCP2 methyl CpG binding protein down-regulates STAT5A and B promoters by interfering with Sp1-induced transcription. In addition, cross-species sequence comparison identified a bi-directional negative cis-acting regulatory element located in the STAT5 intergenic region.

Published
2004
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2. Molecular anatomy of the human glucose 6-phosphate dehydrogenase core promoter.

Author

Franzè A, Ferrante MI, Fusco F, Santoro A, Sanzari E, Martini G, and Ursini MV

Subjects
Animals, Base Sequence, Binding Sites genetics, Cell Line, DNA-Binding Proteins metabolism, DNA-Binding Proteins physiology, Drosophila genetics, Gene Expression Regulation, Humans, Molecular Sequence Data, Mutagenesis, Site-Directed, Nucleic Acid Conformation, Sp1 Transcription Factor metabolism, Sp1 Transcription Factor physiology, Sp3 Transcription Factor, Transcription Factors metabolism, Transcription Factors physiology, Transcriptional Activation, Zinc Fingers, Glucosephosphate Dehydrogenase genetics, Promoter Regions, Genetic genetics
Abstract

The gene encoding glucose 6-phosphate dehydrogenase (G6PD), which plays a pivotal role in cell defense against oxidative stress, is ubiquitously expressed at widely different levels in various tissues; moreover, G6PD expression is regulated by a number of stimuli. In this study we have analyzed the molecular anatomy of the G6PD core promoter. Our results indicate that the G6PD promoter is more complex than previously assumed; G6PD expression is under the control of several elements that are all required for correct promoter functioning and, furthermore, a still unidentified mammalian specific factor is needed.

Published
1998
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