1. Cloning of a human ether-a-go-go potassium channel expressed in myoblasts at the onset of fusion.
- Author
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Occhiodoro T, Bernheim L, Liu JH, Bijlenga P, Sinnreich M, Bader CR, and Fischer-Lougheed J
- Subjects
- Adolescent, Adult, Amino Acid Sequence, Brain metabolism, Cell Differentiation, Cells, Cultured, Child, Child, Preschool, Cloning, Molecular, Ether-A-Go-Go Potassium Channels, Humans, Infant, Molecular Sequence Data, Muscle, Skeletal cytology, Potassium Channels biosynthesis, Sequence Alignment, Sequence Analysis, Muscle, Skeletal metabolism, Potassium Channels genetics
- Abstract
An early sign of human myoblast commitment to fusion is the expression of a non-inactivating delayed rectifier K+ current, I(K(NI)), and an associated membrane potential hyperpolarization. We have isolated the full-length coding region of a human ether-a-go-go K+ channel (h-eag) from myoblasts undergoing differentiation. The h-eag gene was localized to chromosome 1q32-41, and is expressed as a approximately 9 kb transcript in myogenic cells and in adult brain tissue. Forced expression of h-eag in undifferentiated myoblasts generates a current with remarkable similarity to I(K(NI)) indicating that h-eag constitutes the channel responsible for this current in vivo.
- Published
- 1998
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