1. Carboxypeptidase activity in the insulin secretory granule.
- Author
-
Docherty K and Hutton JC
- Subjects
- Animals, Carboxypeptidases antagonists & inhibitors, Hydrogen-Ion Concentration, Rats, Adenoma, Islet Cell enzymology, Carboxypeptidases metabolism, Cytoplasmic Granules enzymology, Insulin metabolism, Insulinoma enzymology, Pancreatic Neoplasms enzymology
- Abstract
Carboxypeptidase activity was studied in subcellular fractions from a transplantable rat insulinoma and found to be localised principally in the insulin secretory granule. The activity, which was specific for peptide substrates with C-terminal basic amino acids, appeared to be a single enzyme with Mr 54000. This enzyme differed with respect to size and pH optimum from other basic amino acid-specific carboxypeptidases, such as carboxypeptidases B and N, and may be a secretory granule-specific enzyme involved in propolypeptide processing.
- Published
- 1983
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