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Your search keyword '"Pussinen, P. J."' showing total 3 results
Start Over Author "Pussinen, P. J." Publisher john wiley & sons
3 results on '"Pussinen, P. J."'

1. Salivary IgA antibody to malondialdehyde–acetaldehyde associates with mild periodontal pocket depth

Author

Akhi, R. (Ramin), Nissinen, A. E. (Antti E.), Wang, C. (Chunguang), Kyrklund, M. (Mikael), Paju, S. (Susanna), Mäntylä, P. (Päivi), Buhlin, K. (Kåre), Sinisalo, J. (Juha), Pussinen, P. J. (Pirkko J.), Hörkkö, S. (Sohvi), Akhi, R. (Ramin), Nissinen, A. E. (Antti E.), Wang, C. (Chunguang), Kyrklund, M. (Mikael), Paju, S. (Susanna), Mäntylä, P. (Päivi), Buhlin, K. (Kåre), Sinisalo, J. (Juha), Pussinen, P. J. (Pirkko J.), and Hörkkö, S. (Sohvi)

Abstract

Objective: Oxidized epitopes such as malondialdehyde–acetaldehyde (MAA) play a crucial role in the progression of atherosclerosis through activation of the humoral immune response. The exact mechanism of the association between atherosclerosis and periodontal diseases is not fully understood. The aim of the current study is to evaluate the association of oral humoral immune response to oxidized epitopes with parameters of periodontal disease. Materials and methods: The Parogene cohort consist of patients who have undergone coronary angiography due to cardiac symptoms. In this study, 423 patients were randomly selected for an extensive oral examination. Salivary Immunoglobulin A to oxidized epitopes and bacterial antigens was determined by chemiluminescence immunoassay. Results: In a binary logistic regression model adjusted with periodontal disease confounders, periodontal pocket depth (PPD) 4–5 mm associated with salivary IgA antibodies to MAA-LDL (p = 0.034), heat shock protein 60 of Aggregatibacter actinomycetemcomitans (p = 0.045), Porphyromonas gingivalis (p = 0.045), A. actinomycetemcomitans (p = 0.005), P. intermedia (p = 0.020), and total IgA (p = 0.003). Conclusions: The current study shows the association of salivary IgA to MAA-LDL with PPD 4–5 mm in a cohort of patients with chronic coronary artery disease. Humoral immune cross-reactivation to oxidized epitopes such MAA-LDL could partly explain the link of periodontitis with systemic diseases.

Published
2022

2. Cross-reactive saliva IgA antibodies to oxidized LDL and periodontal pathogens in humans

Author

Akhi, R. (Ramin), Wang, C. (Chunguang), Kyrklund, M. (Mikael), Kummu, O. (Outi), Turunen, S. P. (S Pauliina), Hyvärinen, K. (Kati), Kullaa, A. (Arja), Salo, T. (Tuula), Pussinen, P. J. (Pirkko J.), and Hörkkö, S. (Sohvi)

Subjects
stomatognathic system, lipids (amino acids, peptides, and proteins)
Abstract

Aim: Oxidized low-density lipoproteins (oxLDL) are formed as a result of lipid peroxidation and are highly immunogenic and proatherogenic. In this study, saliva antibodies binding to oxLDL, Porphyromonas gingivalis (Pg) and Aggregatibacter actinomycetemcomitans (Aa) were characterized and their cross-reactivity was evaluated. Materials and Methods: Resting and stimulated saliva samples were collected from 36 healthy adults (mean age 26 years). Saliva IgA, IgG and IgM autoantibody levels to copper oxidized LDL (CuOx-LDL) and malondialdehyde acetaldehyde-modified LDL (MAA-LDL) were determined with chemiluminescence immunoassay. Results: Saliva IgA and IgG antibodies binding to MAA-LDL and CuOx-LDL were detected in all samples and they were associated with the saliva levels of IgA and IgG to P. gingivalis and A. actinomycetemcomitans. Competitive immunoassay showed that saliva antibodies to MAA-LDL cross-reacted specifically with P. gingivalis. The autoantibody levels to oxLDL in saliva were not associated with the autoantibody levels to oxLDL in plasma or with saliva apolipoprotein B 100 levels. Conclusions: Saliva contains IgA and IgG binding to oxLDL, which showed cross-reactive properties with the periodontal pathogens Porphyromonas gingivalis (P.g). The data suggest that secretory IgA to P.g may participate in immune reactions involved in LDL oxidation through molecular mimicry.

Published
2017

3. Intestinal alkaline phosphatase at the crossroad of intestinal health and disease:a putative role in type 1 diabetes

Author

Lassenius, M. I. (M. I.), Fogarty, C. L. (C. L.), Blaut, M. (M.), Haimila, K. (K.), Riittinen, L. (L.), Paju, A. (A.), Kirveskari, J. (J.), Järvelä, J. (J.), Ahola, A. J. (A. J.), Gordin, D. (D.), Härma, M. (M.‐A.), Kumar, A. (A.), Hamarneh, S. R. (S. R.), Hodin, R. A. (R. A.), Sorsa, T. (T.), Tervahartiala, T. (T.), Hörkkö, S. (S.), Pussinen, P. J. (P. J.), Forsblom, C. (C.), Jauhiainen, M. (M.), Taskinen, M. (M.‐R.), Groop, P. (P.‐H.), Lehto, M. (M.), Lassenius, M. I. (M. I.), Fogarty, C. L. (C. L.), Blaut, M. (M.), Haimila, K. (K.), Riittinen, L. (L.), Paju, A. (A.), Kirveskari, J. (J.), Järvelä, J. (J.), Ahola, A. J. (A. J.), Gordin, D. (D.), Härma, M. (M.‐A.), Kumar, A. (A.), Hamarneh, S. R. (S. R.), Hodin, R. A. (R. A.), Sorsa, T. (T.), Tervahartiala, T. (T.), Hörkkö, S. (S.), Pussinen, P. J. (P. J.), Forsblom, C. (C.), Jauhiainen, M. (M.), Taskinen, M. (M.‐R.), Groop, P. (P.‐H.), and Lehto, M. (M.)

Abstract

Background: Patients with type 1 diabetes have shown an increase in circulating cytokines, altered lipoprotein metabolism and signs of vascular dysfunction in response to high‐fat meals. Intestinal alkaline phosphatase (IAP) regulates lipid transport and inflammatory responses in the gastrointestinal tract. We therefore hypothesized that changes in IAP activity could have profound effects on gut metabolic homeostasis in patients with type 1 diabetes. Methods: Faecal samples of 41 nondiabetic controls and 46 patients with type 1 diabetes were analysed for IAP activity, calprotectin, immunoglobulins and short‐chain fatty acids (SCFAs). The impact of oral IAP supplementation on intestinal immunoglobulin levels was evaluated in C57BL/6 mice exposed to high‐fat diet for 11 weeks. Results: Patients with type 1 diabetes exhibited signs of intestinal inflammation. Compared to controls, patients with diabetes had higher faecal calprotectin levels, lower faecal IAP activities accompanied by lower propionate and butyrate concentrations. Moreover, the amount of faecal IgA and the level of antibodies binding to oxidized LDL were decreased in patients with type 1 diabetes. In mice, oral IAP supplementation increased intestinal IgA levels markedly. Conclusion: Deprivation of protective intestinal factors may increase the risk of inflammation in the gut — a phenomenon that seems to be present already in patients with uncomplicated type 1 diabetes. Low levels of intestinal IgA and antibodies to oxidized lipid epitopes may predispose such patients to inflammation‐driven complications such as cardiovascular disease and diabetic nephropathy. Importantly, oral IAP supplementation could have beneficial therapeutic effects on gut metabolic homeostasis, possibly through stimulation of intestinal IgA secretion.

Published
2017

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