1. [Capillary electrophoresis: principles and practice in clinical laboratory].
- Author
-
Blessum C, Jeppsson JO, Aguzzi F, Bernon H, and Bienvenu J
- Subjects
- Alcoholic Intoxication diagnosis, Amino Acids analysis, Bence Jones Protein urine, Blood Proteins analysis, Carbohydrates analysis, Chromatography, High Pressure Liquid, Chromatography, Micellar Electrokinetic Capillary methods, Fatty Acids analysis, Hemoglobinopathies diagnosis, Hemoglobins analysis, Humans, Immunoglobulins analysis, Isoelectric Focusing methods, Lipoproteins analysis, Nucleic Acids analysis, Pharmaceutical Preparations analysis, Poisoning diagnosis, Proteinuria diagnosis, Transferrin analysis, Electrophoresis, Capillary methods
- Abstract
Capillary electrophoresis represents a relatively new analytical technique. This methodology has diversified and given rise to various modes such as capillary zone electrophoresis, capillary gel electrophoresis, micellar electrokinetic capillary chromatography, capillary isoelectric focusing and capillary isotachophoresis. If capillary electrophoresis was first introduced in research laboratories, this technique is now making an entrance to the clinical laboratory. This is due to its rapid and high-efficiency separation power, its potential applications and its possible automation. Thus, capillary electrophoresis represents an attractive alternative to some time-consuming techniques. Thanks to its versatility, the use of capillary electrophoresis has been proposed for the separation and quantification of a wide spectrum of biological components ranging from macromolecules (proteins, lipoproteins, nucleic acids) to small analytes (amino acids, organic acids or drugs). This paper illustrates the potential of capillary electrophoresis which should rapidly become a major technology for a modern clinical laboratory.
- Published
- 1999