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This study aimed to examine whether discoloration of carious dentin after silver-diammine-fluoride (SDF) application might be used as a mean to detect demineralized-dentin. Fourty specimens were obtained from 20 human permanent teeth. Teeth were sectioned through the center of carious lesions to create 2-halves in which each half was assigned to a treatment group. Specimens were divided into two groups (n=20) (each half was assigned to one group) according to solution, namely Caries Check (CC), or SDF. SDF group was subdivided into 2 groups: light-cured and 2-day storage groups. The specimens were tested using light-microscope, microhardness test and SEM/EDS analysis. Repeated-measures ANOVA was used for statistical analysis. The light-microscope showed superficial discoloration in the CC-group while SDF (2-day storage) group showed deeper discoloration for the lesion area. SDF showed significant increase in the hardness compared with the CC-group. SDF showed potentiality to be used as an assisting-tool for caries detection. [ABSTRACT FROM AUTHOR]