1. Expression and Regulation of Periostin/OSF-2 Gene in Rat Uterus and Human Endometrium
- Author
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Ryo Tsutsumi, Yutaka Osuga, Yumi Hosokawa, Minako Koizumi, Osamu Tsutsumi, Fumiko Nakazawa, Yuji Taketani, Mikio Momoeda, Hisahiko Hiroi, and Tetsu Yano
- Subjects
medicine.medical_specialty ,Time Factors ,Stromal cell ,medicine.drug_class ,Endocrinology, Diabetes and Metabolism ,Integrin ,Cell Culture Techniques ,Periostin ,Rats, Sprague-Dawley ,Andrology ,Endometrium ,Endocrinology ,Internal medicine ,medicine ,Animals ,Humans ,Tissue Distribution ,RNA, Messenger ,Protein kinase B ,Cells, Cultured ,Menstrual Cycle ,Progesterone ,Messenger RNA ,biology ,Chemistry ,Uterus ,Rats ,Gene Expression Regulation ,Cell culture ,Estrogen ,biology.protein ,Immunohistochemistry ,Female ,Stromal Cells ,Cell Adhesion Molecules - Abstract
Periostin/OSF2 is a ligand for alphavbeta3 and alphavbeta5 integrins and activates the Akt/PKB pathway. Recent reports of periostin/OSF2 gene disrupted mice indicate that periostin/OSF-2 plays an important role in implantation. Quantitative RT-PCR revealed that the expression of periostin/OSF-2 mRNA in rat uteri was reduced to approximately 10% at 12 h after 17beta-estradiol (E2) injection, but was not changed after progesterone (P) injection. RT-PCR revealed the expression of periostin/OSF-2 in human endometrium, cultured human endometrial stromal cells (ESCs), and cultured human endometrial epithelial cells. In ESCs, the expression of periostin/OSF-2 mRNA was reduced to approximately 50% at 6 h after E2 treatment. The amount of periostin/OSF2 mRNA in human endometrium significantly increased during mid-proliferative and early secretory phases of menstrual cycle, and decreased during late-proliferative, mid-secretory and late secretory phases. The expression of periostin/OSF2 mRNA significantly decreased in ESCs decidualized by treatment with E2 and P for 7 and 11 days. By immunohistochemistry, the expression of periostin/OSF-2 was strongly detected in endometrial stromal cells during early proliferative, mid-proliferative and early secretory phases, and was strongly detected in endometrial epithelial cells during late secretory phase. This study demonstrated that the expression of periostin/OSF-2 is regulated by ovarian steroid hormones in rat uterus and human endometrium.
- Published
- 2008
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