1. Micromonospora-produced sisomicin components
- Author
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B K, Lee, R G, Condon, G H, Wagman, M J, Weinstein, and E, Katz
- Subjects
Pharmacology ,Time Factors ,Chromatography ,biology ,Bioconversion ,medicine.drug_class ,Chemistry ,Antibiotics ,biology.organism_classification ,Micromonospora ,Anti-Bacterial Agents ,carbohydrates (lipids) ,Methionine ,Biochemistry ,Fermentation ,Drug Discovery ,Sisomicin ,medicine ,Gentamicin ,Gentamicins ,Biotransformation ,medicine.drug - Abstract
A sisomicin fermentation carried out in the presence of (methyl-14C)-L-methionine resulted in a crude mixture, composed of methyl-14C-labeled sisomicin as a major component; and two 4''-C-desmethylsisomicin (66-40B and 66-40D) isomer-like components, an unidentified component and a gentamicin A-like antibiotic as minor components. When (methyl-14C)-L-methionine was added in an early stage of the fermentation (24 hours), incorporation of methyl-14C-label into polar components (e.g., gentamicin A-like antibiotic) preceded that into sisomicin. Chromatographic evidence for the bioconversion of (methyl-14C)-gentamicin A to a radioactive sisomicin-like product (possibly (3''-N-methyl-14C)-sisomicin) was seen, when a Micromonospora blocked mutant was incubated in the presence of the former antibiotic.
- Published
- 1976