1. Detection of SARS-CoV-2 variants related mutations in wastewater using RT-qPCR and variant-specific probes in Porto Alegre, Southern Brazil.
- Author
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Tonietto Mangini, Arthur, Aschidamini Prandi, Bruno, Violet-Lozano, Lina, Ferreira Cunha, Pâmela, Jarenkow, Andre, Scarpellini Campos, Aline Alves, Pasqualim, Gabriela, Hartke, Sara, Brum da Silva, Ilma Simoni, Souza Campos, Fabrício, Michel Roehe, Paulo, and Cláudia Franco, Ana
- Subjects
PATHOGENIC viruses ,SARS-CoV-2 Omicron variant ,SARS-CoV-2 ,POLYMERASE chain reaction ,VIRAL variation - Abstract
The COVID-19 pandemic has underscored the significance of monitoring genetic variations in pathogenic viruses to manage its transmission and identify emerging variants. Nonetheless, sequencing every positive patient case is impractical for large-scale testing of the population. Here, we introduce a cost-effective tool for identifying SARS-CoV-2 mutations associated with variants in wastewater. This approach relies on a wastewater surveillance system and reverse transcription-quantitative polymerase chain reaction (RT-qPCR), utilizing mutation-specific probes. Wastewater samples were analyzed for SARS-CoV-2 mutations corresponding to variants under monitoring (VUM) (P.2, alpha, beta, gamma, delta, and omicron) as part of a wastewater-based SARS-CoV-2 monitoring program. Our findings validate the efficacy of this method in detecting SARS-CoV-2 mutations linked to variants in wastewater, facilitating early detection of emerging variants and informing decision-making on control measures. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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