1. GEP, a Local Growth Factor, is Critical for Odontogenesis and Amelogenesis
- Author
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Zhengguo Cao, Chuan-ju Liu, Yixia Xie, Baichun Jiang, and Jian Q. Feng
- Subjects
Male ,medicine.medical_specialty ,medicine.medical_treatment ,In situ hybridization ,Biology ,dentin ,Applied Microbiology and Biotechnology ,Polymerase Chain Reaction ,Cell Line ,Mice ,Progranulins ,stomatognathic system ,Amelogenesis ,Internal medicine ,medicine ,Ameloblasts ,Animals ,Dental Enamel ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,In Situ Hybridization ,Cell Proliferation ,Mice, Knockout ,Odontoblasts ,Growth factor ,tooth development ,enamel ,Cell Differentiation ,Cell Biology ,Chondrogenesis ,GEP ,DMP1 ,Cell biology ,Odontoblast ,Endocrinology ,Animals, Newborn ,siRNA ,Intercellular Signaling Peptides and Proteins ,Odontogenesis ,Female ,Amelogenin ,Ameloblast ,Developmental Biology ,Research Paper - Abstract
Granulin epithelin precursor (GEP) is a new growth factor that functions in brain development, chondrogenesis, tissue regeneration, tumorigenesis, and inflammation. The goal of this study was to study whether GEP was critical for odontogenesis and amelogenesis both in vivo and in vitro. The in situ hybridization and immunohistochemistry data showed that GEP was expressed in both odontoblast and ameloblast cells postnatally. Knockdown of GEP by crossing U6-ploxPneo-GEP and Sox2-Cre transgenic mice led to a reduction of dentin thickness, an increase in predentin thickness, and a reduction in mineral content in enamel. The in vitro application of recombinant GEP up-regulated molecular markers important for odontogenesis (DMP1, DSPP, and ALP) and amelogenesis (ameloblastin, amelogenin and enamelin). In conclusion, both the in vivo and the in vivo data support an important role of GEP in tooth formation during postnatal development.
- Published
- 2010