Search

The aim of the study was to investigate the dynamics of ultrastructural changes in pulmonary hemocapillaries during experimental diabetes mellitus (DM). Experiments were performed on 40 white male rats Wistar. In the experimental group, diabetes was modeled by intraperitoneal streptozotocin administration, firm «Sigma» (USA), diluted in 0.1 M citrate buffer, pH 4.5, at the rate of 60 mg/kg. Development of the disease was monitored by the glucose levels growth in the animals’ blood. Collecting of lung tissue for electron microscopic examination was performed under general ketamine anesthesia after 1, 2 and 4 weeks after administration of streptozotocin. During the first 2 weeks of the study in the cytoplasm of pulmonary hemocapillaries endothelial was growing the number of micropinocytose vesicules, edema and expansion components of the Golgi complex and granular endoplasmic grid. There can be found erythrocytic sludges in some hemocapillaries. It was found that the most expressed changes in the hemomicrocirculatory channels appear 4 weeks after modeling diabetes. In the endotheliocyte of the pulmonary hemocapillaries there were observed vacuolation of the cytoplasm, swelling of cytoplasmic organelles, thickening of the basement membrane, adhesion and aggregation of blood cells. Thus, studies have shown that experimental diabetes is accompanied by ultrastructural changes of pulmonary hemomicrocirculatory channels components, the severity of which depends on the streptozotocin-induced DM progress.