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This experiment was designed to study the effect of insulin like growth factor-I (IGF-I) on cryopreservation of Hariana bull semen. For this purpose, semen ejaculates were collected from four Hariana bulls using artificial vagina at biweekly interval. The semen samples which possess more than 70% initial progressive motility and above 500 million/mL spermatozoa concentration was subsequently subjected to processing for experiment. Semen samples were extended in egg yolk tris glycerol (EYTG) extender and split into four groups. IGF-I was added at 50 ng/80 × 106 spermatozoa, 100 ng/80 × 106 spermatozoa and 150 ng/80 × 106 spermatozoa in the group II, III, IV respectively and group I as control (without IGF-I). Semen samples were evaluated at pre-freeze and post-thaw stage for percent individual progressive motility, percent live spermatozoa, percent hypo-osmotic swelling (HOS) positive spermatozoa, percent spermatozoa with intact acrosome. A significant (P<0.05) higher individual progressive motility, viability, HOS response and acrosomal integrity was observed at pre-freeze and post-thaw stage by using 150 ng/ml IGF-I. Concentration of 150 ng/mL IGF-I was found to be more beneficial in cryopreservation of Hariana bull spermatozoa as evidenced by post-thaw seminal parameter. It was concluded that IGF-I can be added to extender for improving cryosurvial of Hariana bull spermatozoa. [ABSTRACT FROM AUTHOR]

Different livestock sectors as beef industries get benefit from the production of male animals while dairy industries get benefit from the milk production by the female animals. Therefore, it is obvious need to produce the animals of desired sex which can be achieved by predetermining the sex of conceptus at the time of conception i.e. pre determination of sex may be of great economic importance. Control of the sex ratio by sex prediction of the of pre implanted embryo would be beneficial not only in relation to the aspect of management, production and breeding programmes of livestock but also in diagnosing the genetic disorders at prenatal stage. Pre-implantation sexing of embryos not only improves efficiency of embryo transfer but also facilitate the transfer of embryos of desired sex. Sex-sorted sperm is a one of the technique fulfilling the requirement but it is well expensive and less efficient. Another concept of getting genetically improved animals of desired sex is embryo sexing. Embryo sexing has great potential to maximize the efficiency of dairy production through controlling the sex ratio of domestic species. There are many methods to determine the sex of embryo categorized as invasive and non-invasive techniques with varying efficiency and merits. [ABSTRACT FROM AUTHOR]