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The coastal area of the water and drought graft area and the common area of the performance of two ecosystems with separate characteristics are one another. Coastal environment is a natural evolutionary system that includes the most complex and, at the same time, the richest ecosystems on the planet. This region is a transitional and highly vulnerable region and is endangered by the accumulation of pollutants due to the acceptance of drought and marine pollutants. Marine pollution and the effects of drought activities that are the result of socioeconomic development on the coastline and its catchments are one of the most important issues in most of the world, which directly affects coastal habitats. According to research conducted in Iran on identifying areas and sensitive resources, these areas and resources are divided into two distinct groups: 1) The group of biologically sensitive resources: includes mangrove forests, coral reefs, marine turtles, aquatic birds, marine plants and marine mammals. 2) The group of sensitive physical resources: includes the physical building of beaches in the sub-sections of mud, sand, rocky and hydrographic forms of shores in aquatic, estuarine, clay and small bay. Iranian Fisheries Science Research Institute Published

The Lake Vahdat’s dam ( Kordkandi reservoir ) is located in 75 kilometers eastern of Tabriz in the East of Azerbaijan province With 37°59’ and 46°55’of latitude and attitude ,respectively. The purpose of the Dam is, storage of 6 million m3 of Ojan chai river for aquaculture use in the region. This study was carried out to investigate the possibility of increasing production in the Vahdat’s Dam and some biotic and abiotic factors of lake, such as physico-chemical properties of water, planktons, bentics, icthiology and characteristics of lake's bed were focused. Due to the area of the lake and proximity of the stations to each other, the entrance and estuary of the river as one station and lake as the second station were designated. In the case of phytoplankton, 42 genera were identified from 5 phylom, with 59% Cyanophyta and 21.5% for chlorophylls. For zooplankton, the highest frequency and variety related to the Rotatoria were 50.9% in the spring , which fed by fish and their larvae due to Omega-3 fatty acide. After Rotatoria, Ciliaphora were the most frequent. For Bentics, two sampling steps were carried out in the winter of 2007 and spring of 2008. The high biomass of Tubificidae was showed 0.034 gr ) 84.35/ m3)and 0.42 gr (143.7 /m2) in the winter of 2007 and spring of 2008, respectively. For Ictiology study, the high percentage frequency of fish related to Carassius auratus 42% and Hypophthalmichthys molitrix 38%. The biodiversity of fishes in the lake were confirmed by 12.5 % Alburnus hohenackeri , 87.5 % Carassius gibelio, Carassius auratus, Cyprinus carpio, Hemiculter leucisculus, Hypophthalmichthys molitrix, Hypophthalmichthys nobilis and Ctenopharyngodon idella. One of the most important environmental factors for aquaculture is temperature condition.Now, warm water fish were farmed in the behind of the Dam. The optimal temperature for these fish should be 15-30 ° C. According to the synoptic station of Bostanabad, the temperature of the study area of the Wahdat’s Dam is more than 15 ° C in the four months of the year (from June to September) which is suitable for aquaculture of Cyprinidae. The ice freeze days of the Bostanabad is about 124.6 days , anually. Therefore, the production of warm water fish has been faced with extreme temperature limitation in this lake and only in the short time of the year (mid-spring to end of summer) favorble condition exist for warm water fish farming. Based on the average area of the lake, the production capacity of Cilver Carp and Big head is estimated to be 2.34 and 1.73 tons,respectively.Therefore, assuming of average weight ( 15-20 gr) of fingerlings, 10000 and 7500 number of fingerling shoud be needed for silver carp and big head,repectively.accurding to decresing of variety of bentices founa,increasing of carp production or any other bentic feeder species is needed to manually feeding.In addition to the favorable chemical conditions of water for aquaculture and due to the limited volume of water during summer and suitable temprature of water in spring and fall can farm the fish in the top of the dam. In this regard (HDPEG) cages was established with 750 m3 and 800-1000 number of fish per cge ( 60% cilver carp, 20% big head, 20% carp)with 4.5 ton potential of production. The suitable dimensions of cage are 25-30 diameter, 5 height.There are ten cages fed by artificial food and natural production. Of course there is tourism potential opportunity which can think about that in this area. Iranian Fisheries Science Research Institute Published

The limnological study of the Golabar dam showed that in the Glabar dam inspite of bing its early establishment due to high nutrient and organic matter is located in eutrophic stage. the pH as well as bicarbonate levels shows that buffering capacity of the lake is high and the value of inorganic and organic matter measured are not considered as limiting factor for warme and cold water aquaculture. In the plankton survey 44 species of phytoplankton and 25 species of zooplankton were identified. Cyclotella , Nitzschia, Synedra and Trachelomona from phytoplankton and Polyarthera , Keratella , Filinia , Pompholyx from zooplankton were the dominant spicies.the Bacillariophyta from phytoplankton with 76.5 percent and Rotatoria zooplankton to with 76.2 percent considered the highest abundant . The average frequency of phytoplankton and zooplankton were 5*106 and 723 individual per litter. The Shironomide and Tobificide were the only two bentic group were identified in reservoir wehre their mean frequced were 293.75 and 224.30 respectively. The average biomass of bentic organism were 1.44±0.97 gr/m2.In the survey 12 species of fishes were identified. The potential natural production have been estimated to be 2.8 to 15.5 kg for bentivorous fish and varied from 53 to 175 kg /hec for plankton consumer fishes .The low temperature in several months as well as ice covered of the lake surface in particular in the months of duty and Bahman are the limiting factor of either warm or cold water fish production.

This study focused on population structure, biodiversity, and abundance of the Chitgar Lake fishes in October 2014. Based on the lake location fishes samples were collected at 34 stations in water body. In this study identified 8 family belong to 18 species comprised of Cyprinidae (11 species), Cichlidae (one species), Loricariidae (one species), Pangasiidae (one species), Serrasalmidae (one species), Poeciliidea (one species), Salmonidae (one species), and Scaridae (one species) were Identified. Among the fishes just one native fish from Capoeta bohsei species observed during the study. The dominant lake fishes were invasive species Hemiculter leucisculus, Alburnus hohenackeri, Carassius auratus, Carassius gibelio and Pseudorasbora parva. The highest abundance of fishes were H. leucisculus 62 % and A. hohenackeri 35 % by Seine net. Furthermore, those were dominant abundance (69 and 12 % respectively) in Gill net method. Finally in Cast net method A. hohenackeri 38 %, Pseudorasbora parva 21 % and H. leucisculus 17 % were dominated in the shore region. The lowest fishes abundance were P. hypophthalmus and Parrotfish. Overall more than 90 % of the fishes population were invasive and alien species. Thus, the present of invasive fishes and predator fishes luck in the Chitgar lake might be reason for ecosystem ruin, algae bloom, feed competitive, fish disease and trophy level rises. Iranian Fisheries Science Research Institute Published

The limnological study of the Golabar dam showed that in the Glabar dam inspite of bing its early establishmeant due to high nutrient and organic matter is located in eutrophic stage. the pH as well as bicarbonate levels shows that buffering capacity of the lake is high and the value of inorganic and organic matter measured are not considered as limiting factor for warme and cold water aquaculture.In the plankton survey 44 species of phytoplankton and 25 species of zooplankton were identified.Cyclotella , Nitzschia, Synedra and Trachelomona from phytoplankton and Polyarthera , Keratella , Filinia , Pompholyx from zooplankton were the dominant spicies.the Bacillariophyta from phytoplankton with 76.5 percent and Rotatoria zooplankton to with 76.2 percent considered the highest abundant . the average frequency of phytoplankton and zooplankton were 5*106 and 723 individual per litter. The Shironomide and Tobificide were the only two bentic group were identified in reservoir wehre their mean frequced were 293.75 and 224.30 respectively.The average biomass of bentic organism were 1.44±0.97 gr/m2.In the survey 12 species of fishes were identified.The potential natural production have been estimated to be 2.8 to 15.5 kg for bentivorous fish and varied from 53 to 175 kg /hec for plankton consumer fishes .The low temperature in several months as well as ice covered of the lake surface in particular in the months of duty and Bahman are the limiting factor of either warm or cold water fish production. Iranian Fisheries Science Research Institute Published

This study focused on identification phytoplankton, zooplankton, benthos, fishes and relationship between biotic and abiotic parameters at three stations in the Kan river during 2014. This study identified 19 phytoplankton taxa. The findings showed, the diatoms abundance were dominated in the river. The chlorophytes abundance were recorded the lowest abundance in this study. The nutrients and water temperature were the significant abiotic parameters to increase cyanophytes and chlorophytes abundance in the Kan river. This study identified 26 zooplankton taxa. The Rotatoria and Protozoa abundance were dominated in the Kan river. Based on CCA, there was no correlation between abundance zooplankton and abiotic parameters. The biodiversity index of plankton was between 1.8 and 2 and the lake situation is located in the mesotrophic category. The study of the Kan river fishes showed, only one endemic fish species Capoeta bohsei. The length (between 38 and 270 mm, average 144±86 mm) and weight (between 1.1 and 356 g, average 108.8±146.7 g) of the C. bohsei were varied during the study period. The 19 benthos taxa were identified. The Ephemeroptera was most abundant in the benthos groups. The classification of river based on biology indicator and environmental parameters confimed that water quality is located in the moderate category. Iranian Fisheries Science Research Institute Published

This study focused on identification phytoplankton, zooplankton, benthos, fishes and relationship between biotic and abiotic parameters at three stations in the Kan river during 2014. This study identified 19 phytoplankton taxa. The findings showed, the diatoms abundance were dominated in the river. The chlorophytes abundance were recorded the lowest abundance in this study. The nutrients and water temperature were the significant abiotic parameters to increase cyanophytes and chlorophytes abundance in the Kan river. This study identified 26 zooplankton taxa. The Rotatoria and Protozoa abundance were dominated in the Kan river. Based on CCA, there was no correlation between abundance zooplankton and abiotic parameters. The biodiversity index of plankton was between 1.8 and 2 and the lake situation is located in the mesotrophic category. The study of the Kan river fishes showed, only one endemic fish species Capoeta bohsei. The length (between 38 and 270 mm, average 144±86 mm) and weight (between 1.1 and 356 g, average 108.8±146.7 g) of the C. bohsei were varied during the study period. The 19 benthos taxa were identified. The Ephemeroptera was most abundant in the benthos groups. The classification of river based on biology indicator and environmental parameters confimed that water quality is located in the moderate category.

This study aimed to identify of helminth parasites in some native and economic fish of Anzali wetland was performed . 314 fish specimens, including:Alburnus hohenackeri (60 Specimen), Blicca bjoerkna (75 Specimen), Hemiculter leucisculus (75 Specimen), Pseudorasbora parva (52 pcs) Rhodeus amarus (52 Specimen), from Cyprinidae (Cyprinidae), during 1 year (2012-2013) and seasonally, from three different stations (Shijan, Pirbazar and Pasikhan) via gill net (50-70 mm) and fyke net were sampled. Samples were transported to the Inlandwater Aquaculture Institute in Bandar Anzali. In the laboratory,After biometry and determine the age and sex of fish, different body parts for searching of parasites were checked and parasites by using identification keys were identified. As a result of this study, 9 species of helminth parasites of fishes were identified include: Gyrodactylus sp., Dactylogyrus sphyrna , Dactylogyrus sp., Ligula intestinalis, Diplostomum paraspathaceum, Diplostomum spathaceum, Diplostomum sp., Posthodiplostomum cuticola and Rhabdochona denudate.The highest frequency of parasites in trematodes (in 5 fish species studied) and the minimum frequency in cestodes (each at 1 fish species studied) were seen. According to statistical analysis using Chi-Square test and KruskalWallis, the pollution of the sex, weight, length and sampling stations, there is no statistically significant difference (P> 0.05). Iranian Fisheries Science Research Institute Published

This study aimed to identify of helminth parasites in some native and economic fish of Anzali wetland was performed. 314 fish specimens, including: Alburnus hohenackeri (60 Specimen), Blicca bjoerkna (75 Specimen), Hemiculter leucisculus (75 Specimen), Pseudorasbora parva (52 pcs) Rhodeus amarus (52 Specimen), from Cyprinidae (Cyprinidae), during 1 year (2012-2013) and seasonally, from three different stations (Shijan, Pirbazar and Pasikhan) via gill net (50-70 mm) and fyke net were sampled. Samples were transported to the Inlandwater Aquaculture Institute in Bandar Anzali. In the laboratory, After biometry and determine the age and sex of fish, different body parts for searching of parasites were checked and parasites by using identification keys were identified. As a result of this study, 9 species of helminth parasites of fishes were identified include: Gyrodactylus sp., Dactylogyrus sphyrna , Dactylogyrus sp., Ligula intestinalis, Diplostomum paraspathaceum, Diplostomum spathaceum, Diplostomum sp., Posthodiplostomum cuticola and Rhabdochona denudate. The highest frequency of parasites in trematodes (in 5 fish species studied) and the minimum frequency in cestodes (each at 1 fish species studied) were seen. According to statistical analysis using Chi-Square test and KruskalWallis, the pollution of the sex, weight, length and sampling stations, there is no statistically significant difference (P> 0.05).

This study focused on population structure, biodiversity, and abundance of the Chitgar Lake fishes in October 2014. Based on the lake location fishes samples were collected at 34 stations in water body. In this study identified 8 family belong to 18 species comprised of Cyprinidae (11 species), Cichlidae (one species), Loricariidae (one species), Pangasiidae (one species), Serrasalmidae (one species), Poeciliidea (one species), Salmonidae (one species), and Scaridae (one species) were Identified. Among the fishes just one native fish from Capoeta bohsei species observed during the study. The dominant lake fishes were invasive species Hemiculter leucisculus, Alburnus hohenackeri, Carassius auratus, Carassius gibelio and Pseudorasbora parva. The highest abundance of fishes were H. leucisculus 62 % and A. hohenackeri 35 % by Seine net. Furthermore, those were dominant abundance (69 and 12 % respectively) in Gill net method. Finally in Cast net method A. hohenackeri 38 %, Pseudorasbora parva 21 % and H. leucisculus 17 % were dominated in the shore region. The lowest fishes abundance were P. hypophthalmus and Parrotfish. Overall more than 90 % of the fishes population were invasive and alien species. Thus, the present of invasive fishes and predator fishes luck in the Chitgar lake might be reason for ecosystem ruin, algae bloom, feed competitive, fish disease and trophy level rises.

A mass-balance Ecopath model of coastal areas in the Caspian Sea has been constructed for a quantitative description of the trophic structure of the ecosystem. The model is used to estimate the important biological parameters and relationships among the different ecologically important groups. Twenty five species were used in the present analysis. The model is based on the data collected of Kilka fisheries, beach seining (Bony fish fisheries), and set Gillnet (Sturgeon Fisheries) and also dietary information. Total landings along the Iranian portion of southern Caspian coast reached 39647 t, including sturgeon (41 t), kilka (22873 t) and bony fishes (16733 t) in 2014. Two species of kilka (Common kilka and Anchovy) are important commercially in the Caspian Sea, together accounting in the past decade for > 60 % of the total catch, as well as being a crucial part of the food chain. The mean average trophic level was estimated at 3.17 by Ecopath software. In this research, the mean level were studied between eight species varied from 2.56 to 4.04, Sander lucioperca occupy the highest and the as vimba vimba was the lowest level. The ranges of total mortality varied from 0.5 to 2.56 per years. The food consumption rate was estimated about 101.56 per year Mixd trophic level index (MTI) showed small pelagic fishes as prey, have crucial role in feeding of pelagic predators population such as invasion of the ctenophore Mnemiopsis leidyi, bream fish and benthic fish such as sturgeon. In general, niche overlap was greater in more species such as Alburnus chalcoides, Rutilus rutilus, Clupeonella cultriventris, Rutilus kutum and M. leidyi who consumed large amounts of plankton. In conclusion small pelagic fish exert a major control on the trophic dynamics of the Caspian Sea ecosystems and constitute midtrophic-level populations.

The aim of this study was to find the best location for establishment of rainbow trout culture and hatcheries farms on Zayanderood’s river region in Charmahal and Bakhtiyari province. This survey carried out over ten station along Zayanderood’s river. The result of physical and chemical analysis showd that the annual average of air temperature varied from 9.5oc o 10oc wehre the pH annual average value were between 7.5 and 8.8. the dissolve oxygen concentration in stations except in rainbow trout farm effluent were above 10 mg/l. the other chemical , pollutant as well as pesticides levels were under the limiting concentration for rainbow trout culture and Hatcheries activity . The plankton survey showed that the Bacillurophyta were the dominant group of phytoplankton where protozoa constituted the most abundant group of zooplankton the Benthic organisms sensitive to pollutant in particular Epirus were dominant group in all stations . In regard to fishes presence in river , five species of Ciprinidae , one species from Balitoridae and one species from salmonidae families were identified . The capacity for development of rainbow trout culture for tow phase period in Zayanderood’s river region with respect to self purification potential (self purification potential were determined from the oxidation of the effluent of the only active trout farm of the river region), minimum of 10 L/s water requirement for production of trout in concrete canal and pond system and 1L/S water need for production in semi circular closed system were estimated to be 5202 metric tons. Iranian Fisheries Science Research Institute Published

The aim of this study was to find the best location for establishment of rainbow trout culture and hatcheries farms on Zayanderood’s river region in Charmahal and Bakhtiyari province. This survey carried out over ten station along Zayanderood’s river. The result of physical and chemical analysis showd that the annual average of air temperature varied from 9.5oC - 10oC where the pH annual average value were between 7.5 and 8.8. the dissolve oxygen concentration in stations except in rainbow trout farm effluent were above 10 mg/l. the other chemical , pollutant as well as pesticides levels were under the limiting concentration for rainbow trout culture and Hatcheries activity. The plankton survey showed that the Bacillurophyta were the dominant group of phytoplankton where protozoa constituted the most abundant group of zooplankton the Benthic organisms sensitive to pollutant in particular Epirus were dominant group in all stations. In regard to fishes presence in river, five species of Ciprinidae, one species from Balitoridae and one species from salmonidae families were identified. The capacity for development of rainbow trout culture for tow phase period in Zayanderood’s river region with respect to self purification potential (self purification potential were determined from the oxidation of the effluent of the only active trout farm of the river region), minimum of 10 L/s water requirement for production of trout in concrete canal and pond system and 1 L/S water need for production in semi circular closed system were estimated to be 5202 metric tons.

Caspian trout (Salmo trutta caspius ) is one of the migratory fish species which lives and feeds in the Caspian Sea .This species distributed in south and western of the Caspian Sea . In order to rehabilitation of the Caspian trout in western area, the operation of the project started from the beginning of the fishing season of the 20112012. Specimens of the Caspian trout obtained from beach seine cooperatives and have been transferred to the fish farm in Asalem region .Totally, 44 specimens of the Caspian trout has been caught in two month period .Most of the fishes was from Kargan roud river . The mean length and weight of the brood fishes were 67.7 cm and 3122.7 g respectively .As at present Shilat uses just the broods of the Tonekaboon region for restocking of this species ,we recommend to use the broods of the western region separately for rehabilitation of the stocks of this region .

Genetic knowledge helps to protect biodiversity and optimal harvest resources by several ways. This knowledge can help to reduce the risk of extinction to those populations that have high genetic diversity or diminished by detected them. Also, studies of population genetics can present guidelines for improving the structure of the population and understanding the biology of species. One of the most important applications of DNA database is detecting species, fishing offense, diagnose of anemia and genetic classification of animals. DNA bank has helped to identify the species that are hunted and were discovered only parts of their meat and texture. Optimal Storage of aquatic genetic resources and the conservation of biological diversity, as the national capital by using biotechnology methods are the most important goals of gene bank and database reserves of the country creation. Collecting biological specimens of aquatic species, preparation and long-term maintenance of them, genetic registration of endangered and threatened species and the use of biotechnology techniques for the protection, conservation and management of aquatic genetic resources is one of the other functions of the gene bank. Gene bank of inland waters of Iran (Gorgan) is planned and implemented to identify, collect, maintain and protect species at the level of genes, cells, tissues and live fish research and commercial exploitation. Despite the predicted projected funding for this project, unfortunately funding sources have been considered, does not allocated. Therefore some parts of the aims of this project has been organized by Golestan province credits to build molecular laboratory and aquaculture pond in Alang farm and exploitation of potential Gharasou station and Sijoval center restocking and the conditions for the bulk of the project goals is provided. Some parts like tissue culture laboratory and bioinformatics as well as parts and equipment related to the freezing of sperm and embryo development are the items that should be considered in the development of the this bank.

Tish survey prolonged from summer 2011 trough summer 2012. During this period 574 samples of different fish species were investigated for their parasites. Totally 30 species of parasites isolated of the fishes. Some of the isolated parasites are recorded for the first time in Iran. Rafidascaris acus Orientocreadium siluri, Silurotaenia siluri, Acanthocephalus lucii , Argulus foliaceus has recorded for the first time from European catfish, Silurus glanis, and Dactylogyrus inexpectatus has recorded for the first time from Gibel Carp, Carassius auratus gibelio, in Anzali wetland. Statistical comparison of parasites infections and intensity between the different area of Anzali wetland were done. Infection of Pike, Esox lucius, to Diplostomum spathaceum, Raphidascaris acus and Monogenean in western parts of wetland were significantly different from the Eastern and Central areas (p

Caspian trout (Salmo trutta caspius ) is one of the migratory fish species which lives and feeds in the Caspian Sea .This species distributed in south and western of the Caspian Sea . In order to rehabilitation of the Caspian trout in western area, the operation of the project started from the beginning of the fishing season of the 20112012. Specimens of the Caspian trout obtained from beach seine cooperatives and have been transferred to the fish farm in Asalem region .Totally, 44 specimens of the Caspian trout has been caught in two month period .Most of the fishes was from Kargan roud river . The mean length and weight of the brood fishes were 67.7 cm and 3122.7 g respectively .As at present Shilat uses just the broods of the Tonekaboon region for restocking of this species ,we recommend to use the broods of the western region separately for rehabilitation of the stocks of this region . Iranian Fisheries Science Research Institute Published

Tish survey prolonged from summer 2011 trough summer 2012. During this period 574 samples of different fish species were investigated for their parasites. Totally 30 species of parasites isolated of the fishes. Some of the isolated parasites are recorded for the first time in Iran. Rafidascaris acus Orientocreadium siluri, Silurotaenia siluri, Acanthocephalus lucii , Argulus foliaceus has recorded for the first time from European catfish, Silurus glanis, and Dactylogyrus inexpectatus has recorded for the first time from Gibel Carp, Carassius auratus gibelio, in Anzali wetland. Statistical comparison of parasites infections and intensity between the different area of Anzali wetland were done. Infection of Pike, Esox lucius, to Diplostomum spathaceum, Raphidascaris acus and Monogenean in western parts of wetland were significantly different from the Eastern and Central areas (p

The Goal of project implementation was survey of biological roles of Northern pike, Esox lucius, in decreasing of Coarse fish and other un economics organisms in carps ponds and also increasing of final crops in hectare and determination of Suitable ration of pike in ponds. This project implemented by 5 treatment with 3 replicates for every ones in 2 years. The pike density was 200, 350, 500 and 650 fish individuals per hectare for 1, 2, 3 and 4 treatment, respectively, and fifth treatment was without pike. The treatments of 1 and 3 conducted in first year and other in second year of test period. At first, about 26 brooders Caught from Anzali Lagoon, and reproduced by artificial and semi - natural methods in Sefidroud Fisheries Research Station in 12–14 °c temperature. Produced larvae reared in earthen ponds and fries reached to weight of 10 -12 g after 45 days. Mean weight, length and survival rates of fish was 12.27 and 32.5 g, 11.44 and 15.83 cm, and 40 and 21% after 45 and 60 days, respectively. The carp density in ponds was 3500 fish individuals per hectare and stocking rates were 55 % silver carp, 20 % common carp, 10 % bighead carp and 15 % grass carp. In throughout of the experiment period, physico– chemical factors measured and plankton (phyto and zoo), benthos, un wanted fish and other organisms investigated. The biomass of unwanted fishes reduced to 76/81 and 60/6 % in first and second year of experiments respectively. The results showed that Esox lucius had effect in increasing of objective fish produce about 17.9 % and 3.9 % in first and second period of project implementation respectively. The average of fish produce in pond unit (2400 m^2)was 842 kg (3508 kg/ha ), 825 kg (3439 kg/ha) and 776 kg (3232 kg/ha) in first , second and control treatments first year of test period , and also in second year was 865 kg (3603 kg/ha), 877 kg (3652 kg/ha) and 848 kg (3531 kg/ha) in second , fourth and control treatments , respectively. A significant different found in final weight mean of common carp between treatments and Control ponds (P

Anzali wetland at south west of Caspian located along of North West and southeast and with its unique feature were add on the Ramsar convention list in 1975. There were recorded a high rich of fauna and flora including 276 plant species, 23 reptiles and amphibians, 60 fishes, 26 mammals and 195 birds. Anzali wetland were encountered with numerous disorder with the eutrophication and the early dismissing. The first studies on Anzali wetland started by many international institutions in 1964. In this study the data of biotic and abiotic factors in Anzali wetland analyzed which belongs to different sites (15 to 38 stations) during 1991 to 2000 and were sampled seasonally or monthly in different years. The results showed the increasing trend of phytoplankton abundance from 7.9 to 66 million C./l. and the western part had the most abundant. The Cyanophyta had the most development than other 7 phylum observed in the area. In Anzali wetland identified 143 phytoplankton genus that 9 genus dominated in more than 80 % replicates and 7 genus had the abundance of more than 5 million cell/l. The Zooplankton abundant increased from 580 to 2200 ind./l. The Rotatoria had the most abundant followed by Copepoda. There was identified 104 zooplankton genus that 5 genus were dominated in more than 80 % replicates and 11 genus had the abundance of more than 30 ind./L. The chlorophyll a varied from 8.8 to 50.2 μg/l. and had a increasing trend after 1996, meanwhile it was measured higher than 25 μg/l in most of the stations. The results of benthic study showed that the Chironomidae and Tubificidae were observed more than other organisms which comprised 12.2 to 23.5% of total biomass of benthic organisms in studied period. The range of biomass was 1.1 to 7.8 g/m2. The ichthyologic survey showed presence of 60 species that 11 species were exotic. 28 species belong to freshwater, 17 species were anadromous and 8 species were resident in estuary. The highest and lowest fish catch were 640 and 288 ton in 1994 and 1999 respectively and it was about 500 ton at the end of period. The hydrochemical factors had the various fluctuation so that the nutrients parameters had a significant difference over studied period. Total nitrogen was varied from 0.78 to 1.41 mg/l and total phosphorus varied from 0.05 to 0.1 μg/l.. The oxygen dissolved amount was from 4.6 to 8.7. EC was between 3 to 5 ms/cm during September and October that indicated of Caspian water projection. According to geographic position of station, all biotic and abiotic data, were arranged. The data bank is used by Idrisi software to produce of necessary maps. Analysis of data showed that there must be a responsible department with a vast authority. Conservation and eutrophication prevention of Anzali wetland connect to special attention to catchment area and landuse management of the wetland. Also there is an urgent need for well-developed technologies and managements to reduce the organic pollution and its environmental impacts on the Anzali Lagoon.

The Goal of project implementation was survey of biological roles of Northern pike, Esox lucius, in decreasing of Coarse fish and other un economics organisms in carps ponds and also increasing of final crops in hectare and determination of Suitable ration of pike in ponds. This project implemented by 5 treatment with 3 replicates for every ones in 2 years . The pike density was 200, 350, 500 and 650 fish individuals per hectare for 1 , 2 , 3 and 4 treatment , Respectively, and fifth treatment was without pike. The treatments of 1 and 3 conducted in first year and other in second year of test period. At first, about 26 brooders Caught from Anzali Lagoon, and reproduced by artificial and semi - natural methoods in Sefidroud Pisheries Research Station in 12–14 °c temperature. Produced larvae reared in earthen ponds and fries reached to weight of 10 -12 g after 45 days. Mean weight, length and survival rates of fish was 12.27 and 32.5 g , 11.44 and 15.83 cm , and 40 and 21% after 45 and 60 days , respectively . The carp density in ponds was 3500 fish individualsper hectar and stocking rates were 55 % silver carp, 20 % common carp , 10 % bighead carp and 15 % grass carp . In throughout of the experiment period, physico – chemical factors measured and plankton ( phyto and zoo) , benthos , un wanted fish and other organisms investigated . The biomass of unwanted fishes reduced to 76/81 and 60/6 % in first and second year of experiments respectively. The results showed that Esox lucius had effect in increasing of objective fish produce about 17.9 % and 3.9 % in first and second period of project implementation respectively . The average of fish produce in pond unit ( 2400 m2 )was 842 kg ( 3508 kg/ha ), 825 kg ( 3439 kg/ha ) and 776 kg ( 3232 kg/ha ) in first , second and control treatments first year of test period , and also in second year was 865 kg ( 3603 kg/ha ), 877 kg ( 3652 kg/ha) and 848 kg ( 3531 kg/ha) in second , fourth and control treatments , respectively . A significant different found in final weight mean of common carp between treatments and Control ponds (P

The present study was conducted to survey the artificial spawning of European catfish (Silurus glanis L.) by synthetic hormones include Carp pituitary extract (CPE), Ovo fact [D-Ala6 des Gly10] GnRHa Ethyl amid in combination with domperidone diluted in propylene glycol, Human gonadotropin chryonic hormone (HCG) and LHRHa (des-Gly10, [D-Ala6] LH-RH Ethyl amide). In this research work 44 male and 72 female brood stokes that transferred to the Shaheed Dr Ansari hatchery and Sefid rod research station were studied on 2 years and injected with those stimulated maturation. Total length, total weight, gonad weight were measured to the nearest 0.1 cm, 1g and 0.01g respectively. The experiments were conducted using different doses of hormones on 4 treatments. In treatment CPE that brood stocks injected with 2.5, 3, 4 and 5 mg/kg/BW, the most responded (83.33%) was with 5mg/kg/Bw of CPE. The mean fertilization in this treatment was 92.5 4.51 and after that there are groups which were stimulated by 4 mg/kg/BW of CPE .In treatment ovafact that brood fish were injected by 10, 20, 30, 40 μg/kg/BW of this stimulation hormone showed 100 percent of fish ovulated in the group treated with 40 μg/kg/BW of ovofact. The mean fertilization rate was 86.08 6.27 in this group. 33.3 % of brood fish ovulated in the group that injected with 40 μg/kg/BW of LHRHa stimulation, but none of brood stock reached to spawning with injection of 30 μg/kg/BW of LHRHa. The brood stocks were injected by 800, 850, 900, 950 IU/kg/BW of HCG, only 33.3 percent of fish in groups that received 900 & 950 IU/kg/BW of this Stimulation hormone reached to spawning stages. According to our results and statistical analysis between trial treatments in percent of Embryo viability showed significant difference (P

Anzali wetland at south west of Caspian located along of North West and southeast and with its unique feature were add on the Ramsar convention list in 1975. There were recorded a high rich of fauna and flora including 276 plant species, 23 reptiles and amphibians, 60 fishes, 26 mammals and 195 birds. Anzali wetland were encountered with numerous disorder with the eutrophication and the early dismissing. The first studies on Anzali wetland started by many international institutions in 1964. In this study the data of biotic and abiotic factors in Anzali wetland analyzed which belongs to different sites (15 to 38 stations) during 1991 to 2000 and were sampled seasonally or monthly in different years. The results showed the increasing trend of phytoplankton abundance from 7.9 to 66 million C./l. and the western part had the most abundant. The Cyanophyta had the most development than other 7 phylum observed in the area. In Anzali wetland identified 143 phytoplankton genus that 9 genus dominated in more than 80 % replicates and 7 genus had the abundance of more than 5 million cell/l. The Zooplankton abundant increased from 580 to 2200 ind./l. The Rotatoria had the most abundant followed by Copepoda. There was identified 104 zooplankton genus that 5 genus were dominated in more than 80 % replicates and 11 genus had the abundance of more than 30 ind./L. The chlorophyll a varied from 8.8 to 50.2 μg/l. and had a increasing trend after 1996, meanwhile it was measured higher than 25 μg/l in most of the stations. The results of benthic study showed that the Chironomidae and Tubificidae were observed more than other organisms which comprised 12.2 to 23.5% of total biomass of benthic organisms in studied period. The range of biomass was 1.1 to 7.8 g/m2. The ichthyologic survey showed presence of 60 species that 11 species were exotic. 28 species belong to freshwater, 17 species were anadromous and 8 species were resident in estuary. The highest and lowest fish catch were 640 and 288 ton in 1994 and 1999 respectively and it was about 500 ton at the end of period. The hydrochemical factors had the various fluctuation so that the nutrients parameters had a significant difference over studied period. Total nitrogen was varied from 0.78 to 1.41 mg/l and total phosphorus varied from 0.05 to 0.1 μg/l.. The oxygen dissolved amount was from 4.6 to 8.7. EC was between 3 to 5 ms/cm during September and October that indicated of Caspian water projection. According to geographic position of station, all biotic and abiotic data, were arranged. The data bank is used by Idrisi software to produce of necessary maps. Analysis of data showed that there must be a responsible department with a vast authority. Conservation and eutrophication prevention of Anzali wetland connect to special attention to catchment area and landuse management of the wetland. Also there is an urgent need for well-developed technologies and managements to reduce the organic pollution and its environmental impacts on the Anzali Lagoon. Iranian Fisheries Science Research Institute Published

Mnemiopsis leidyi is one the species of comb-jelly. It belongs to the Ctenophore phylum and Lobate order. It is a purely marine, free-swimming animal with a transparent jelly-like walnut-shaped body, which was transported from the Black Sea into Caspian possibly at the end of 1990s. This species is food rival of pelagic fish. It has been reported that the ctenophore caused the dramatic decrease of zooplankton and pelagic fish stock in the Black Sea. In this study, sampling on Mnemiopsis leidyi, zooplankton, phytoplankton was conducted from December 2003 to March 2005, from a total of 11 stations located along four transects (Anzali, Khazarabad, Tourkman and Gorgan Bay) by METU net in the Iranian coasts of the Caspian Sea. Clupeonella catch was done by light fishing with fishing vessel in Anzali region. The results showed that the biomass of M.leidyi had some fluctuation in different seasons and its maximum biomass was recorded in summer with a figure of 524.4±156.2 g.m^2 Tourkman region and the minimum biomass was observed in winter with a figure of 5.15±1.81 g.m^2 in Anzali region. Study of stomach of Mnemiopsis showed, that Acartia (belonged of Copepoda with 66 %), lamlibranchia (13 %) has the highest frequency and the lowest were Balanus nauplii, Rotatoria, Tintinnopsis and Podon polyphemoides. The maximum mean abundance of zooplankton was 29368±24318 n.m3 in winter (Anzali region) and the minimum was recorded 4170±5014 n.m3 in summer (Tourkman region). Copepoda was dominant zooplankton in the Iranian coasts of the Caspian Sea. Chrysophyta with 150000000±440000000 n.m3 and Euglenophyta with 260000±521000 n.m3 had the highest and the lowest frequency respectively in the Caspian Sea. The main foods of Clupeonella were Acartia with 80 %. The survey of stomach of Clupeonella showed that the feeding of these species was not good and the highest fullness index was recorded 220.95±314.67. These results showed, the main food item of Ctenophora and Clupeonella were Copepoda (Acartia). It seems, the impact of Mnemiopsis leidyi feeding has been the important factor in declining zooplankton populations, Clupeonella stocks, and also increasing of phytoplankton abundance.

Mnemiopsis leidyi is one the species of comb-jelly. It belongs to the Ctenophore phylum and Lobate order. It is a purely marine, free-swimming animal with a transparent jelly-like walnut-shaped body, which was transported from the Black Sea into Caspian possibly at the end of 1990s. This species is food rival of pelagic fish. It has been reported that the ctenophore caused the dramatic decrease of zooplankton and pelagic fish stock in the Black Sea. In this study, sampling on Mnemiopsis leidyi, zooplankton, phytoplankton was conducted from December 2003 to March 2005, from a total of 11 stations located along four transects (Anzali , Khazarabad, Tourkman and Gorgan Bay) by METU net in the Iranian coasts of the Caspian Sea. Clupeonella catch was done by light fishing with fishing vessel in Anzali region. The results showed that the biomass of M.leidyi had some fluctuation in different seasons and its maximum biomass was recorded in summer with a figure of 524.4±156.2 g.m2 Tourkman region and the minimum biomass was observed in winter with a figure of 5.15±1.81 g.m2 in Anzali region. Study of stomach of Mnemiopsis showed, that Acartia (belonged of Copepoda with 66 %), lamlibranchia (13 %) has the highest frequency and the lowest were Balanus nauplii, Rotatoria, Tintinnopsis and Podon polyphemoides. The maximum mean abundance of zooplankton was 29368±24318 n.m3 in winter (Anzali region) and the minimum was recorded 4170±5014 n.m3 in summer (Tourkman region). Copepoda was dominant zooplankton in the Iranian coasts of the Caspian Sea. Chrysophyta with 150000000±440000000 n.m3 and Euglenophyta with 260000±521000 n.m3 had the highest and the lowest frequency respectively in the Caspian Sea. The main foods of Clupeonella were Acartia with 80 %. The survey of stomach of Clupeonella showed that the feeding of these species was not good and the highest fullness index was recorded 220.95±314.67. These results showed, the main food item of Ctenophora and Clupeonella were Copepoda (Acartia). It seems, the impact of Mnemiopsis leidyi feeding has been the important factor in declining zooplankton populations, Clupeonella stocks, and also increasing of phytoplankton abundance. Iranian Fisheries Science Research Institute Published

This project has been started as monitoring study since 2001 that in first phase has lasted for two years and because of its importance, it was decided that this study should be continued for two other years. In the early 1980s, an alien ctenophore Mnemiopsis leidyi already known as a gelatinous zooplankton was transported (likely via ballast waters) to the Black Sea from its native waters of the western Atlantic. The possibility of Mnemiopsis leidyi introduction into other sensitive, neighboring ecosystems, notably the Caspian Sea, had been mentioned during the GESAMP meeting in 1994. And, as expected, this ctenophore was reported to be present in the Caspian Sea by November 1999. In this report, spatial and temporal distribution of M. leidyi had been investigated in southern Caspian Sea (Iranian waters) in 6 transects consisted of Lisar, Anzali, Sefidroud, Nowshar, Babolsar and Amirabad at 24 stations during 2003 and 2004. As a whole, 818 samples had been analyzed included 236, 302, 136 and 144 belong to ctenophore, phytoplankton, zooplankton and benthos. Meantime 811 physicochemical parameters had been measured. Water temperature varied between 7.8 to 29.3 co. Transparencies also fluctuated from 0.20 to 10.5 m. Salinity in different regions was from 7.80 to 13.90 ppt and the average was 12.09 ppt. pH was between 7.2- 8.6. Demand oxygen (O2) has been measured very high in water surface (12.89 µg.l) and it decreased at minimum level in deep down to 4 µg.l. Nutrients measurements such as NO_2, NO_3 and NH_4 were 0.10-15.17 , 3.80- 133.40 and 0.36- 86.40 µg.l, respectively. Total nitrate and phosphate were measured between 337.00- 1635.00 and 14.90-103.40 µg.l, respectively. Comparison of M. leidyi distribution in the southern Caspian Sea shown that maximum abundance recorded in warm summer months (e.g. August) with 11627 ind.m^2 and its biomass maximum was in autumn months (September and October) about 446.11 gr.m^2 . Mean abundance and biomass of M. leidyi were 2504 ind.m^2 and 142.42 gr.m^2, respectively. Maximum size of the ctenophore recorded as 70 mm while the less than 10 mm length frequency consisted 92.2 per cent of total population. Spatial distribution of M. leidyi in different regions shown that Sefidroud not only had the maximum Mnemiopsis abundance but also consisted higher biomass in the southern Caspian Sea in 2003-4. At present study, 142 phytoplankton species were identified which belongs to Chrysophyta (67, 48), Chlorophyta (23, 16%), Cyanophyta (22, 15%), Pyrhophyta (21, 15%) and Euglenophyta (9, 6%). Mean phytoplankton abundance and biomass were 11137032 ind. m^3 and 40.26 mg. m^3, respectively. Chrysophyta (diatoms) consisted 42 and 47% of whole phytoplankton abundance and biomass. In the other hand, this group included 50 % of phytoplankton population. In zooplankton study, there were identified 21 species in which 10 species were only meroplankton (zoobenthic) and 11 species was holoplankton. These species belongs to Copepoda (4, 37%), Rotatoria (4, 36%), Cilliophora (2, 18%) and Cladocera (1, 9%). Monthly zooplankton abundance and biomass data shown that its population completely effected by Copepoda. Among copepoda, Acartia clausi was the main species duri ng 2003-4. As a whole, maximum abundance recorded in spring (March) and autumn (September) about 11497 and 11321 ind. m^3 while its maximum biomass occurred in summer (August) with 48.95 mg. m^3. Zooplankton minimum abundance and biomass were in wintertime about 3894 ind. m^3 and 10.16 mg. m^3. Benthic fauna in recent study consisted 27 species included Gammaride (8, 29%), Cumacea (7, 26%), Polychaeta (4, 15%), Bivalvia (3, 11%) and Oligochaeta, Balanidae and Xantidae each one (4%) species. Mean benthic abundance and biomass were 1215 ind. m^ 2 and 20.09 g. m^2, respectively. Polychaeta and Oligochaeta were established maximum frequency about 49 and 34% of total fauna while Bivalvia consisted 82.8% biomass overall.

This project has been started as monitoring study since 2001 that in first phase has lasted for two years and because of its importance, it was decided that this study should be continued for two other years. In the early 1980s, an alien ctenophore Mnemiopsis leidyi already known as a gelatinous zooplankton was transported (likely via ballast waters) to the Black Sea from its native waters of the western Atlantic. The possibility of Mnemiopsis leidyi introduction into other sensitive, neighbouring ecosystems, notably the Caspian Sea, had been mentioned during the GESAMP meeting in 1994. And, as expected, this ctenophore was reported to be present in the Caspian Sea by November 1999. In this report, spatial and temporal distribution of M. leidyi had been investigated in southern Caspian Sea (Iranian waters) in 6 transects consisted of Lisar, Anzali, Sefidroud, Nowshar, Babolsar and Amirabad at 24 stations during 2003 and 2004. As a whole, 818 samples had been analized included 236, 302, 136 and 144 belong to ctenophore, phytoplankton, zooplankton and benthos. Meantime 811 physicochemical parameters had been measured. Water temperature varied between 7.8 to 29.3 co . Transparencies also fluctuated from 0.20 to 10.5 m. Salinity in different regions was from 7.80 to 13.90 ppt and the average was 12.09 ppt. pH was between 7.2- 8.6. Demand oxygen (O2) has been measured very high in water surface (12.89 µg.l) and it decreased at minimum level in deep down to 4 µg.l. Nutrients measurements such as NO2, NO3 and NH4 were 0.10-15.17 , 3.80- 133.40 and 0.36- 86.40 µg.l, respectively. Total nitrate and phosphate were measured between 337.00- 1635.00 and 14.90-103.40 µg.l, respectively. Comparison of M. leidyi distribution in the southern Caspian Sea shown that maximum abundance recorded in warm summer months (e.g. August) with 11627 ind.m 2 and its biomass maximum was in autumn months (September and October) about 446.11 gr.m 2 . Mean abundance and biomass of M. leidyi were 2504 ind.m2 and 142.42 gr.m 2 , respectively. Maximum size of the ctenophore recorded as 70 mm while the less than 10 mm length frequency consisted 92.2 per cent of total population. Spatial distribution of M. leidyi in different regions shown that Sefidroud not only had the maximum Mnemiopsis abundance but also consisted higher biomass in the southern Caspian Sea in 2003-4. At present study, 142 phytoplankton species were identified which belongs to Chrysophyta (67,48 ), Chlorophyta (23,16%), Cyanophyta (22,15% ), Pyrhophyta (21,15% ) and Euglenophyta (9,6%). Mean phytoplankton abundance and biomass were 11137032 ind. m 3 and 40.26 mg. m 3 , respectively. Chrysophyta (diatoms) consisted 42 and 47% of whole phytoplankton abundance and biomass. In the other hand, this group included 50 % of phytoplankton population. In zooplankton study, there were identified 21 species in which 10 species were only meroplankton (zoobenthic) and 11 species was holoplankton. These species belongs to Copepoda (4,37% ), Rotatoria (4,36% ), Cilliophora (2,18% ) and Cladocera (1,9% ). Monthly zooplankton abundance and biomass data shown that its population completely effected by Copepoda. Among copepoda, Acartia clausi was the main species duri ng 2003-4. As a whole, maximum abundance recorded in spring (March) and autumn (September) about 11497 and 11321 ind. m 3 while it s maximum biomass occurred in summer (August) with 48.95 mg. m 3 . Zooplankton minimum abundance and biomass were in wintertime about 3894 ind. m 3 and 10.16 mg. m 3 . Benthic fauna in recent study consisted 27 species included Gammaride (8,29% ), Cumacea (7, 26% ), Polychaeta (4,15% ), Bivalvia (3,11% ) and Oligochaeta, Balanidae and Xantidae each one (4% ) species. Mean benthic abundance and biomass were 1215 ind. m 2 and 20.09 g. m 2 , respectively. Polychaeta and Oligochaeta were established maximum frequency about 49 and 34% of total fauna while Bivalvia consisted 82.8% biomass overall. Iranian Fisheries Science Research Institute Published

For gathering of data on biometry and catch statfstics, 5, mobile working group in the regions of Anzali , Kiashahr , Noshahr , Babolsar and Torkaman . Were involved from the start to the end of fishing season. After gathering,data Were intered to the computer based on species. For data analysis different methods Were undertaken sach as Bertalanfy equation for growth parameter Beverton and Holt for total mortality rates, pauly's imperical model for natural mortality rate and cohort Analysis for biomas estimation. The total recorded Catch in 2001 and 2002 was 10529 and 9566 tonnes. Totall catch with taking into account of pauching . estinmated 16200 and 15200 tonnes .total catch of Katum in 2002 had 1200 tonnes decrease with comparison of last year. Biomass of kutum in IRanian coastal Water of the Caspian sea in 2002 estimated about 15000tonnes. The catch of golden mullet in 2001 and 2002 was estimated 4218 and 6183 tonnes.that about 29%-38% of the catch of this species was undersize .The biomass of golden mullet estimated about 16400 tonnes. The small nose mullet in 2002 comprise 9% of mullet catches. The catches of common carp in Anzali region was very low .but stocks of this species in Gorgan region is in better condition which.Most of catches of this species were fished illgally in the Gorgan bay and Gomishan coastal creek. The biomass of this species was estimated 1140 tonnes Most of catches of the roach.were fished illgally .Totall catches of this species in 2002 were estimated about 738 tonnes. The catch of pickperch in 2002 were estimated about 30 tonnes.Most of the chatch of pickperch was immature and undersize. Appooximattelly all of the catch is due to releasing of fingerling of this species by fishery company. Totall catch of bream in 2002 was estimated 38 tonnes that most of which was immature and undersize. Iranian Fisheries Science Research Institute Published