1. Coupling gold nanoparticles to silica nanoparticles through disulfide bonds for glutathione detection
- Author
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Mei-Jin Li, Kar Seng Teng, Heng Zhang, Zhaomin Zhang, Zhenfeng Yue, Changqing Yi, Yupeng Shi, and Mengsu Yang
- Subjects
Nanostructure ,Materials science ,Surface Properties ,Static Electricity ,Bioengineering ,Nanotechnology ,Photochemistry ,Electron spectroscopy ,Fluorescence spectroscopy ,Spectroscopy, Fourier Transform Infrared ,Zeta potential ,General Materials Science ,Disulfides ,Electrical and Electronic Engineering ,Fourier transform infrared spectroscopy ,Spectroscopy ,Photoelectron Spectroscopy ,Mechanical Engineering ,General Chemistry ,Silicon Dioxide ,Glutathione ,Spectrometry, Fluorescence ,Mechanics of Materials ,Covalent bond ,Colloidal gold ,Nanoparticles ,Spectrophotometry, Ultraviolet ,Gold - Abstract
Advances in the controlled assembly of nanoscale building blocks have resulted in functional devices which can find applications in electronics, biomedical imaging, drug delivery etc. In this study, novel covalent nanohybrid materials based upon [Ru(bpy)3](2+)-doped silica nanoparticles (SiNPs) and gold nanoparticles (AuNPs), which could be conditioned as OFF-ON probes for glutathione (GSH) detection, were designed and assembled in sequence, with the disulfide bonds as the bridging elements. The structural and optical properties of the nanohybrid architectures were characterized using transmission electron microscopy, UV-vis spectroscopy and fluorescence spectroscopy, respectively. Zeta potential measurements, x-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy were employed to monitor the reaction processes of the SiNPs-S-S-COOH and SiNPs-S-S-AuNPs synthesis. It was found that the covalent nanohybrid architectures were fluorescently dark (OFF state), indicating that SiNPs were effectively quenched by AuNPs. The fluorescence of the OFF-ON probe was resumed (ON state) when the bridge of the disulfide bond was cleaved by reducing reagents such as GSH. This work provides a new platform and strategy for GSH detection using covalent nanohybrid materials.
- Published
- 2013