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Microfluidic delivers miniaturized fluidic networks for processing liquids in the microliter range. In the recent years, lab-on-chip (LOC) is become a main tool for point-of-care (POC) diagnostic especially in the medical field. In this paper, we presented a design and fabrication on multi disease analysis using single chip via delivery of fluid with the multiple transducers is the pathway of multi-channel microfluidic based LOC’s. 3 in 1 nano biosensor kit was attached with the microfluidic to produce nano-biolab-on-chip (NBLOC). The multi channels microfluidic chip was designed including the micro channels, one inlet, three outlet and sensor contact area. The microfluidic chip was designed to include multiplex detection for pathogen that consists of multiple channels of simultaneous results. The LOC system was designed using Design Spark Mechanical software and PDMS was used as a medium of the microfluidic. The microfluidic mold and PDMS microfluidic morphological properties have been characterized by using low power microscope (LPM), high power microscope (HPM) and surface profiler. The LOC system physical was experimental by dropping food coloring through the inlet and collecting at the sensor contact area outlet.

The aim of this research is to study the morphological analysis of fabricated Interdigitated Electrode (IDE). This device electrode was physically characterized using 3D nano profiler, scanning electrode microscope (SEM), Energy-dispersive X-ray spectroscopy (EDX) and Atomic Force Microscope (AFM). Based on this analysis, IDE pattern was analyzed thoroughly based on the IDE pattern specifications with 5 μM finger gap and this research significantly will stand as a platform quantify the biomolecules in further analysis.

This paper focuses on the ongoing development of real-time monitoring system with implementing the Internet of Things (IoT) element for arsenic heavy metal concentration in paddy field using pH sensor for data collection. The pH sensor will detect the hydrogen ion concentration from the prepared soil put in pot contained with various arsenic concentration. The developed system is then compared with the pH sensor in the market to verify its accuracy and sensitivity. The collected data will be transferred wirelessly into data cloud so that it can store the previous and current reading data. Besides, the system’s function is also to ensure the safeness of paddy plant to be planted with knowing the right amount of arsenic concentration can be passed.

According to recent studies, millions of people are suffering from Arsenic (As) toxicity contamination. Arsenic is one of the most toxic and carcinogenic chemical elements and its contamination in paddy field has become a chronic problem. In the agricultural sector, arsenic-contaminated water irrigation has a higher level of arsenic contamination on the top soil, which can affect the quality of crop production such as rice plants. Rice plant requires a considerable amount of water to complete its lifecycle. In Asean countries like Malaysia, Indonesia and Thailand, Arsenic intake from rice is significantly higher, as rice plants have a special ability to take up arsenic from the soil and water used for irrigation. The transfer of arsenic from soil to plant systems is a serious issue that leads to considerable human exposure. Nanotechnology application plays vital role for any field work and research. In this research, the implementation of nanotechnology approach such as silica nanoparticles are able to remove the Arsenic heavy metal contamination. This paper focused on arsenic heavy metal removal using silica nanoparticles analaysed using spectrophotometric method.

Abundant resources of rice straw and carbon-neutral have a lot of potentials to be converted into various value-added product and become more green due to less utilization of chemical usage. Graphene is one of value-added had been converted from rice straw and was successfully synthesized by using activating rice straw ash (RSA) using potassium hydroxide (KOH) at 700 oC with 1:2.5 impregnation ratio. scanning electron microscopy (SEM), field emission scanning electron microscopy (FESEM), EDX Analysis with a field emission transmission electron Microscope (FETEM) and atomic force microscopy (AFM). The current results can be the model for comparing the synthesize graphene from other plant sources.

Turning waste product into the valuable resources is the best alternative way to overcome the waste management issue. Generally, rice is grown and planted twice a year where a lot of rice by-products have been produced after harvesting the matured paddy. Rice straw is one of turning waste products into the valuable resources and to manage the environmental issues. Generally, rice is grown and planted twice a year where a lot of rice by-products are produced. Rices straw is one of the rice by-products, generated roughly 0.7-1.4 kg per kilograms of harvested milled rice. With the nanotechnological approach, silica particles at nano-size can be produced using the incinerated rice straw. In addition to that, this research will report the synthesis, characterization and adsorption analysis towards the heavy metal removal.

The aim of the research study to design high sensitive biosensor for medical applications. IDE pattern was designed using AutoCAD software with 5 µm ginger gap. The fabrication process was done using a conventional photolithography process and standard CMOS process. The fabricated electrode was physically characterized using a low power microscope (LPM) and a high power microscope (HPM). The electrically validated through I-V measurements and chemically tested with different pH buffer solutions. Al IDE was well fabricated with 0.1 µm tolerance between the design mask and fabricated IDEs. Electrical measurements confirmed that IDE was well fabricated without any shortage and results of similar IDE samples were confirmed that the repeatability of the device. The extremely small current variations in nano ampere range were quantitatively detected using an extra small volume of 2 µl for different pH buffer solutions. It is confirmed that IDEs are sensitive in both alkali and hydroxyl ions medium.

This research was developed to focus on the study of the voltammetric DNA biosensor for the detection of HPV strain 18. In this research, electrical DNA biosensor was expected to detect HPV strain 18 more efficiently by using electrical characterization. In this project, device inspection was conducted to make sure the functional of the gold interdigitated electrode (IDE) by using Scanning Electron Microscope (SEM). 3-Aminopropyl Triethoxysilane (APTES) solution was used for the process of surface modification to form the amine group on the surface of the device to facilitate the attachment of the DNA probe. In this project, synthetic DNA sample and DNA from the saliva of several Biosystems Engineering students were used as the target DNA. The current-voltage (I-V) electrical characterization was conducted to detect the presence of HPV strain 18 in both DNA samples. As the results, perfect alignment between the electrodes on the IDE was detected under SEM. Surface modification of the biosensor successfully conducted which is the covalent bond between APTES and DNA probe increase the electrical. Synthetic DNA shows the presence of HPV strain 18 while there was no HPV strain 18 detected in the DNA from saliva samples.

The aim of this work was to develop a novel, simple, inexpensive, sensitive an electrochemical DNA biosensor based on interdigitated electrodes (IDEs) integrated gold nanoparticle modified electrode by electro less deposition for HPV 18. The biosensor was designed with a 30 mer E6 region of HPV 18 DNA modified probe. The E6 region has been used for their clinical importance properties and suitable as recognition biomarker region. Three different target types were tested which complementary target, non-complementary target and mismatch target. All target were analyzed for detection of HPV 18 in early stages by using Dielectric Analyzer (DA), Alpha-A High-performance Frequency Analyzer, Novocontrol Technologies, Handsagen, Germany associated with the software package Windeta. Complementary target gives a positive result in HPV detection, while non-complementary and mismatch target give negative results. IDE device with 5 nm gap sizes has demonstrated a high performance towards the detection of HPV18 ssDNA target by modified with 30 nm gold nanoparticle. The electrochemical biosensor showed better performance compared to agarose gel electrophoresis assay. This technology can be used as a new and attractive sensor development for detection of virus infection in human bodies.

A novel coronavirus (2019-nCoV) became the seventh member in the family of Coronavirus that infect human. 2019-nCoV became the most severe virus compare to another family of coronavirus. Human airway ephitelial cells have been used to identify and isolate the virus before proceed to reverse trancriptase Polymerase Chain Reaction (RT-PCR). Detailed biological knowledge is crucial for the development of effective countermeasures, diagnostic tests, vaccines and antiviral drugs against the 2019-nCoV. Conserved coding sequences within the spike glycoprotein region of open reading frame in the coronavirus genome was used as the basis to design oligonucleotide probe to detect the virus. Analyses on different strain of coronavirus sequences were done to check the percentage of similarity and consensus region that cause different strain of viruses. The biomarker needed an acceptable length between 22 and 31 mers. The choice of S gene region was identified and can be used as a biomarker probe for biosensor development. It has implications for coronavirus detection techniques in clinical and biosensor diagnostic system.

The agriculture sector plays an important role in economic development in providing rural employment, uplifting rural incomes and ensuring national food security. Harumanis mango has been predominantly produced in the Perlis state of Malaysia, and it is the seasonal once-a-year. However, plant diseases became a serious issue that significantly affects both the quality and quantity of Harumanis mango production. The primary problem with Harumanis mango has experienced by farmers is a disease and the farmers are failed to complete the process of post-harvest for Harumanis mango. Further, in the case of Harumanis mango disease, the symptoms are often detected at the later stage, which makes it difficult to take any further action. Usually, the diseases are controlled by fungicides and the use of synthetic chemicals to eliminate the postharvest diseases and deterioration has their limit due to carcinogenicity, teratogenicity, environmental pollution, effects on food and other side-effects on humans. Up to date, the control of Harumanis mango disease is still challenging, although several studies have been conducted to eradicate and prevent its outbreak in the field. This overview is about Harumanis mango and the prospect in preventing a serious outbreak with Harumanis mango production using interdigitated electrodes (IDE) nano-biosensor.

The persistent infection by high risk HPV is a necessary but not sufficient cause of this cancer which develops over a long period through precursor lesions which can be detected by electrochemical impedance sensor. The HPV driven molecular mechanisms underlying the development of cervical lesions have provided a number of potential biomarkers for both diagnostic and prognostic use in the clinical management of women with HPV related cervical disease and these biomarkers can also be used to increase the positive predictive value of current methods. The most influential methods for the detection and identification of HPV using gold nanoparticle (GNP) included electrochemical impedance sensor will visit their sensitivity, selectivity and characteristic detection on synthetic target which are complement of the DNA, non-complement of the DNA and mismatch of the DNA. In difference concentration of synthetic target, which stage can get the exactly value to determine the HPV in strain 16 was evaluated in this research studies.

In Malaysia, the first Iodine Deficiency Disorders (IDD) survey was conducted in 1996 and it was discovered that Peninsular Malaysia did not have IDD problem until latter studies showed goitre occurrence of 34.7% in Hulu Langat district and urinary iodine lower than the adequate level of (100-199 ug/L) in Perak and Pahang states (Selamat et al., 2010). Baseline and periodical sampling of children and pregnant woman urine and imported salt commodities for the consumption of the population is mandatory for iodine measurement. Thus, development of quantitative methods of measurement of salt and food iodine is crucial for implementation of the USI program nationwide. In this study, interdigitated electrode (IDE) biosensor, a rapid, sensitive and selective method has been developed to determine the iodine content in both urine and salt. This method includes functionalization and silanization step using 3-aminopropyl triethoxysilane (APTES). The I-V characterization of IDE biosensor was performed using (Keithley 2450), Kickstart software and Probestation. It measures the amount of current flow through IDE which is directly proportional to the concentration of iodine in both urine and salt. Hence, IDE biosensor is proven to be a rapid, selective, sensitive method and can be developed as a new nanotechnology for the elimination of Iodine Deficiency Disorders (IDD) among children and pregnant woman.

Amplification of nano and mircoampere electrical signal to the detectable range is essential in the biosensor field. This research is mainly focused on design an amplifier circuit to capture and amplify three different range of current as nano, micro and mili ampere and convert it to detectable voltage range as an output signal to the processing circuit. The Proteus 8 Pro software was used to design, simulate and calibrate the amplifier circuit. Firstly, current input as mili, micro and nano current were flown through 0.1 mΩ, 10 Ω and 10 KΩ resistors, respectively to convert different current inputs to the similar range in micro voltage. The MAX 4238 opamp IC was used to amplify micro voltage to mili voltage. LM 358 dual operational amplifier was used to supply virtual ground to MAX 4238 amplifier. The amplified output voltage of three different current inputs as nano, micro and mili were nearly equal to theoretical outputs.

Contamination of various food samples became one of the critical issues in food pathogen infection. Food pathogen can be detected by using digital polymerase chain reaction (PCR) and sequencing. These methods were reliable but consuming and take a longer time for detection. The present work describes the innovation to develop a technology to extract double-stranded deoxyribonucleic acid (dsDNA) from food samples and then denatured dsDNA into and single-strand DNA (ssDNA) for further use on the chip using microfluidic device. Microfluidic device is a lab-on-chip device that consist of microfluidic channels that provide paths for biomolecules to flow to individual point of care. DNA extraction is the process by which DNA is separated from proteins, membranes, and other cellular material contained in the cell from which it is recovered. Lysis solution is used in the process of extraction the DNA to break up the cells containing DNA from protein and other cellular materials. This extraction firstly be done in the most labour-intensive in obtaining the DNA biomolecules. Extraction methods may require an overnight incubation, may be a protocol that can be completed in minutes or a couple of hours by using a commercial kit. The disadvantages of the laboratory and commercial kit is due to time-consuming, poor cost-effectiveness, the need to use big laboratory and a complicated process which need an expertise to conduct the experiment and interpret the data. This research is proposed to design and fabricate a microfluidic device that has DNA extraction capabilities. In this research DNA extraction using a commercial kit will be used as a comparison for the quality of the result. The microfluidic device can be used in health care delivery system and will help the doctors in diagnostic process to identify disease of a patient rapidly. Other than that, the output extracted from microfluidic device will be used for DNA probe target interaction for diagnostic kit. The major advantage of microfluidic device is that it consumes less time compared to the conventional chemical methods.

According to media reports in today’s world, millions of people across the globe are suffering from arsenic (As) toxicity. Arsenic heavy metal occurs in different oxidative states in the environment and enters in the food chain through soil and water. In the agricultural field, irrigation with arsenic contaminated water has a higher level of arsenic contamination on the top soil, which may eventually affects the quality of crop production. The major crop like rice requires a considerable amount of water to complete its lifecycle. In Asian countries like Malaysia, Brunei, Indonesia, and Thailand, arsenic intake from rice diet is significantly higher, as rice plants have a special ability to take up arsenic from the soil and water used in irrigation. The transfer of arsenic from soil to plant systems is a serious issue that leads to considerable human exposure in a dangerous way. The applications of nanotechnology play a vital role in many research fields. And therefore, this research, employs the implementation of nanotechnology approach such as silica-grapheme nanocomposite that could be able to minimize the Arsenic heavy metal contamination issues.

Silicon carbide is a promising material for various applications, especially for applications in high frequency, high power and high temperature and harsh conditions such as highly oxidative and corrosive environment. Currently, silicon carbide is produced by conventional heating process. For silicon carbide nanomaterials, various methods have been attempted. In this study, SiC nanowhiskers were synthesized by microwave heating of blends of graphite and silica in the ratio of 3: 1. Graphite of two different size, namely micro graphite and nano graphite were used. The blends firstly prepared by ultrasonic mixing graphite and silica in ethanol followed by drying. The blends were then heated by using laboratory microwaves furnace to 1450 °C at heating rate of 20 °C/min for 20 minutes. Finally, the SiC nanowhiskers was characterized by using scanning electron microscope.