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Dryobalanops belong to a member of Dipterocarpaceae, which has a high economic value. Due to illegal harvesting for its wood, the population tends to decrease every year, so rapid conservation effort was needed. D. oblongifolia and D. lanceolata were important members of dryobalanops, which have listed as endangered (EN), according to IUCN. This study aims to provide information on the DNA isolation and amplification process. This data was needed as basic information for further research, such as genetic diversity, population genetics, phylogeny studies, or biogeography aspects. DNA isolation was performed using the CTAB method (Cetyl Trimethyl Ammonium Bromide). The amplification process was performed with three barcode markers those were trnL-trnF, rbcL, and matK. The results of DNA isolation showed quite good DNA yield. Amplified DNA using all primers showed successfully with an annealing temperature of 490C for matK, 500C for trnL-trnF, and 550 C for rbcL.

The existence of Dryobalanops sumatrensis and D. oblongifolia subsp. oblongifolia in Indonesia’s natural forest is increasingly threatened due to land use changes and illegal logging leading to the urgent need of conservation actions in the field. Vegetative propagation by shoot cuttings has been proposed as an alternative method, yet there have been still lacks of information regarding the suitable rooting media and the use of shoots from saplings as cutting materials. This study was to evaluate the survival rate, rooting and shoot sprouting abilites of D. sumatrensis and D. oblongifolia subsp. oblongifolia on two different media (mineral soil and peat). Saplings of the D. sumatrensis aged older than 7 years old and D. oblongifolia subsp. oblongifolia aged less than 2 years old were used as cutting materials. The results showed that the survival rate of D. sumatrensis in peat (43.75%) and mineral soil media (43.75%) was lower than D. oblongifolia subsp. oblongifolia in for peat media (54.55%) and minerals soil media (71.88%). The rooting and shoot sprouting percentage of D. sumatrensis in peat and mineral soil media was also lower than D. oblongifolia subsp oblongifolia. This pattern revealed that the media treatment (peat and minerals) did not affect the survival rate and rooting ability of D. sumatrensis shoot cuttings, but has an effect on D. oblongifolia subsp. oblongifolia.