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Acute exposure to high dose of ultraviolet (UV) radiations is known to cause significant harm to skin, primarily due to the generation of free radicals and damage to DNA, which often culminate in rapid aging of the skin, or cancers. Keratinocytes being the most abundant skin’s cells are affected most by UV. Although a degree of endogenous protection is present, the vulnerability of UV-induced damaged can be minimized using protective agents. A few UV filters (organic and inorganic) have been successfully commercialized, yet, due to prevailing disadvantages such as low solubility, photostability, and aesthetic sense, suitable and more efficient UV filters continue to be explored as potential ingredients of cosmaceutical agents. A recently studied antioxidant enzyme mimetic cerium oxide nanoparticles showed emerging piece of evidence on benefits under environmental stress. However, its protective abilities as potential UV filter and therefore applicability in cosmaceutical has not yet been completely explored. This study provides a piece of evidence in support of beneficial effects of this new class of UV filters, polyethyleneglycol functionalized nanoceria (PEG-CNP) against UV - induced damage in vitro and in vivo. The nanomolar concentration of PEG-CNPs in the cell culture showed significant protection from UV exposure, by direct ROS scavenging, the rescue of cells from cell cycle arrest and DNA damage. Further, a proof of the concept study in dehaired rat skin showed that the topical application of 50 μM PEG-CNPs prevented the initial signs of UV induced damage. Unlike conventional UV filters, PEG-CNPs confer protection by internalizing the cells, and scavenging the radicals.

'Exercise' is a double-edged sword for the skeletal muscle. Small amount of ROS generated during mild exercise, is essential for normal force generation; whereas large quantity of ROS generated during intense exercise, may cause contractile dysfunction, resulting in muscle weakness and fatigue. One of the key question in skeletal muscle physiology is 'could antioxidant therapy improve the skeletal muscle endurance? A question, which has resulted in contradictory experimental findings till this date. This work has addressed this 'very question' using a synthetic, inorganic, antioxidant nano-material viz., 'cerium oxide nanozyme' (CON). It has been introduced in the rat by intramuscular injection, and the skeletal muscle endurance has been evaluated. Intramuscular injections of CON, concurrent with exercise, enhanced muscle mass, glycogen and ATP content, type I fiber ratio, thus resulting in significantly higher muscle endurance. Electron microscope studies confirmed the presence of CON in the vicinity of muscle mitochondria. There was an increase in the number and size of the muscle mitochondria in the CON treated muscle, following exercise, as compared to the untreated group with only exercised muscle. Quantitative proteomics data and subsequent biological network analysis studies, identified higher levels of oxidative phosphorylation, TCA cycle output and glycolysis in CON supplemented exercised muscle over only exercised muscle. This was further associated with significant increase in the mitochondrial respiratory capacity and muscle contraction, primarily due to higher levels of electron transport chain proteins like NDUFA9, SDHA, ATP5B and ATP5D, which were validated by real-time PCR and western blotting. Along with this, persistence of CON in muscle was evaluated with ICP-MS analysis, which revealed clearance of the particles after 90 d, without exhibiting any inflammation or adverse affects on the health of the experimental animals. Thus a higher physiological endurance of the CON supplemented exercised muscle' opens new avenues in skeletal muscle therapeutic, space and sports medicine.