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The title compound, C24H46N4O82+·2Cl−·2H2O, was synthesized by the hydrolysis of tetraethyl 2,2′,2′′,2′′′-(5,5,7,12,12,14-hexamethyl-1,4,8,11-tetraazacyclotetradecane-1,4,8,11-tetrayl) tetraacetate in hydrochloric acid solution. The crystal structure of the title compound consists of a 14-membered C10N4 centrosymmetric cationic macrocycle which interacts with the chloride ions and water molecules of crystallization to give a three-dimensional hydrogen-bonded network.