1. Apoptosis-inducing Effect of a Palladium(II) Complex-[PdCl(terpy)](sac).2H2O] on Ehrlich Ascites Carcinoma (EAC) in Mice.
- Author
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Ikitimur-Armutak EI, Ulukaya E, Gurel-Gurevin E, Yaylim I, Isbilen-Basok B, Sennazli G, Yuzbasioglu-Ozturk G, Sonmez K, Celik F, Kucukhuseyin O, Korkmaz G, Yilmaz VT, and Zeybek SU
- Subjects
- Animals, Antineoplastic Agents pharmacology, Carcinoma, Ehrlich Tumor drug therapy, Carcinoma, Ehrlich Tumor metabolism, Cell Proliferation drug effects, Cisplatin pharmacology, Coordination Complexes chemistry, Drug Therapy, Combination, Female, Immunoenzyme Techniques, Mice, Mice, Inbred BALB C, Apoptosis drug effects, Apoptosis Inducing Factor pharmacology, Carcinoma, Ehrlich Tumor pathology, Coordination Complexes pharmacology, Palladium chemistry
- Abstract
Background/aim: New compounds for cancer treatment are needed due to persistenly unsatisfactory management of cancer. [PdCl(terpy)](sac)·2H2O] (sac=saccharinate, and terpy=2,2':6',2"-terpyridine) is a compound synthesized for this purpose. We investigated its anti-proliferative and pro-apoptotic effects on Ehrlich Ascites Carcinoma (EAC) in vivo., Materials and Methods: 42 Balb-c female mice were subcutaneously (s.c.) injected with EAC cells (1st day) and then randomly divided into 5 groups: control (0.9% NaCl), complex (2 mg/kg), complex (3 mg/kg) cisplatin (4 mg/kg) and paclitaxel (12.5 mg/kg). On the 5th and 12th day animals were drug administrated. At 14th day, animals were sacrificed. Expression of cell death and/or cell cycle-related markers (Bcl-2, Bax, active caspase-3, p53, PCNA) and apoptosis were investigated immunohisto-chemically. Survival-related markers (Akt, GSK-3β, IGF-1R, IR, IRS-1, p70S6K, PRAS40) were evaluated by luminex analysis., Results: Expression of p53, PCNA, Bcl-2 was found decreased (p<0.001) and that of active caspase-3, Bax, and apoptotic cells was found increased (p<0.001) in all groups. The survival-related markers did not show any statistical difference in complex groups., Conclusion: The Pd(II)-complex seems to have a strong anticancer activity on EAC by inducing apoptosis via both suppression of proliferation and activation of apoptosis in vivo, similar to the effects of cisplatin and paclitaxel., (Copyright © 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.)
- Published
- 2016