1. Synergistic Effect of Apigenin and Curcumin on Apoptosis, Paraptosis and Autophagy-related Cell Death in HeLa Cells.
- Author
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Kayacan S, Yilancioglu K, Akdemir AS, Kaya-Dagistanli F, Melikoglu G, and Ozturk M
- Subjects
- Adaptor Proteins, Signal Transducing genetics, Caspase 3 physiology, Cell Survival drug effects, Drug Synergism, Endoplasmic Reticulum Chaperone BiP, Female, HeLa Cells, Heat-Shock Proteins analysis, Humans, Membrane Proteins genetics, Proto-Oncogene Proteins genetics, Uterine Cervical Neoplasms drug therapy, Apigenin pharmacology, Apoptosis drug effects, Autophagic Cell Death drug effects, Curcumin pharmacology
- Abstract
Background/aim: We aimed to investigate the synergistic effects of apigenin and curcumin on the cross-talk between apoptosis and autophagic cell death, as well as on paraptosis in HeLa cells., Materials and Methods: Cell viability was measured using the MTT assay. Synergistic effects were measured using the Bliss independence model. qRT-PCR was used to study the expression of genes related to apoptosis, autophagic cell death, and cross-talk. GRP78/BiP immunostaining was used to identify endoplasmic reticulum (ER) stress., Results: Treatment with a combination of apigenin and curcumin increased the expression levels of genes related to cell death in HeLa cells 1.29- to 27.6-fold. The combination of curcumin and apigenin showed a synergistic anti-tumor effect via cross-talk between processes leading to apoptosis and autophagic cell death, as well as ER stress-associated paraptosis. GRP78 expression was down-regulated, and massive cytoplasmic vacuolization was observed in HeLa cells., Conclusion: The combination of curcumin and apigenin is an effective potential therapeutic for cervical cancers., (Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Published
- 2021
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