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Keywords: IgY antibody – Tetanus toxoid – Antibody stability – ELISA – Clostridium tetani. Submitted by ROBERTO PAULO CORREIA DE ARAÚJO (ppgorgsistem@ufba.br) on 2017-06-01T19:14:32Z No. of bitstreams: 1 10_v.8_3.pdf: 390371 bytes, checksum: fec8c68a5bd6eddfbad5af57220fddfd (MD5) Made available in DSpace on 2017-06-01T19:14:32Z (GMT). No. of bitstreams: 1 10_v.8_3.pdf: 390371 bytes, checksum: fec8c68a5bd6eddfbad5af57220fddfd (MD5) Previous issue date: 2009-09 Abstract: Tetanus is an acute, occasionally a fatal, disease of the central nervous system, caused by the toxin of the tetanus bacterium Clostridium tetani. The current techniques used in the diagnosis and therapeutics of tetanus diseases employ monoclonal antibodies produced in mouse. The use of monoclonal antibodies has several advantages such as homogeneity and specificity. In contrast, a remarkable characteristic of polyclonal antibodies, especially the immunoglobulin class IgY, which is extracted from poultry, is obtained in greater amount than IgG antibodies. Therefore, the alternative production of IgY polyclonal antibodies against tetanus toxoid in chicken can become of great interest for immunology industry in therapeutic and diagnosis. In this work, IgY polyclonal antibodies against tetanus toxoid were produced through immunization of chickens with isolated toxoid and measured by enzyme-linked immunosorbent assay (ELISA). The effect of storage temperature on the stability of chicken IgY was measured through indirect ELISA. Results showed that the IgY antibodies were produced with success and easily isolated. The time and temperature influence was measured through indirect ELISA. Altogether, the results indicate the use IgY polyclonal antibodies purified from chicken as an alternative for the detection of the tetanus toxin with potential applications in diagnosis and treatment of infectious diseases. Salvador

Keywords: IgY antibody – Tetanus toxoid – Antibody stability – ELISA – Clostridium tetani. Submitted by ROBERTO PAULO CORREIA DE ARAÚJO (ppgorgsistem@ufba.br) on 2017-06-01T19:14:32Z No. of bitstreams: 1 10_v.8_3.pdf: 390371 bytes, checksum: fec8c68a5bd6eddfbad5af57220fddfd (MD5) Made available in DSpace on 2017-06-01T19:14:32Z (GMT). No. of bitstreams: 1 10_v.8_3.pdf: 390371 bytes, checksum: fec8c68a5bd6eddfbad5af57220fddfd (MD5) Previous issue date: 2009-09 Abstract: Tetanus is an acute, occasionally a fatal, disease of the central nervous system, caused by the toxin of the tetanus bacterium Clostridium tetani. The current techniques used in the diagnosis and therapeutics of tetanus diseases employ monoclonal antibodies produced in mouse. The use of monoclonal antibodies has several advantages such as homogeneity and specificity. In contrast, a remarkable characteristic of polyclonal antibodies, especially the immunoglobulin class IgY, which is extracted from poultry, is obtained in greater amount than IgG antibodies. Therefore, the alternative production of IgY polyclonal antibodies against tetanus toxoid in chicken can become of great interest for immunology industry in therapeutic and diagnosis. In this work, IgY polyclonal antibodies against tetanus toxoid were produced through immunization of chickens with isolated toxoid and measured by enzyme-linked immunosorbent assay (ELISA). The effect of storage temperature on the stability of chicken IgY was measured through indirect ELISA. Results showed that the IgY antibodies were produced with success and easily isolated. The time and temperature influence was measured through indirect ELISA. Altogether, the results indicate the use IgY polyclonal antibodies purified from chicken as an alternative for the detection of the tetanus toxin with potential applications in diagnosis and treatment of infectious diseases. Salvador

Keywords: Canine distemper virus – Egg yolk antibody – ELISA, indirect. Submitted by ROBERTO PAULO CORREIA DE ARAÚJO (ppgorgsistem@ufba.br) on 2016-09-15T17:17:02Z No. of bitstreams: 1 3_v.8_1.pdf: 219352 bytes, checksum: 3e8590d89e26705803a41ad72bf1d3b2 (MD5) Made available in DSpace on 2016-09-15T17:17:02Z (GMT). No. of bitstreams: 1 3_v.8_1.pdf: 219352 bytes, checksum: 3e8590d89e26705803a41ad72bf1d3b2 (MD5) Previous issue date: 2009-01 Abstract: Canine distemper is a contagious, incurable, often fatal, multisystemic viral disease that affects the respiratory, gastrointestinal, and central nervous systems. Distemper is caused by the canine distemper virus (CDV). The development of antibodies to use in therapy and diagnosis is essential for the control of some diseases. Immunization of chickens with CDV results in the production of antibodies specific. However, there is relatively limited information available concerning immune response of CDV in this species. In the present study, immune responses were examined in serum and egg yolk from laying hens injected with CDV. The results demonstrated that the increase of antibody activity occurs first in the serum, and then in egg yolk with a lag in time of 1 to 3 week in the chickens. However, the time of elevated levels of antibody activity was much shorter in serum than the egg yolk. Salvador

Keywords: Canine parvovirus - Diagnosis - Egg yolk antibody- ELISA, indirect; Canine parvovirus - Diagnosis - HA-HI Submitted by ROBERTO PAULO CORREIA DE ARAÚJO (ppgorgsistem@ufba.br) on 2016-09-15T16:43:52Z No. of bitstreams: 1 4_v.7_3.pdf: 214631 bytes, checksum: c74d99fe9749dea1e8fb6759c7a3c70a (MD5) Made available in DSpace on 2016-09-15T16:43:52Z (GMT). No. of bitstreams: 1 4_v.7_3.pdf: 214631 bytes, checksum: c74d99fe9749dea1e8fb6759c7a3c70a (MD5) Previous issue date: 2008-09 Canine parvovirus (CPV), cause an intestinal disease characterized by bloody diarrhea, is often fatal in puppies. The virus is transmitted by contact with infected dogs or their feces. The virus is very stable in the environment and may survive for several months in contaminated areas. The CPV attacks the rapidly diving cells of the bone marrow and the small intestine. Several laboratory tests have been developed and are available for specific viral diagnosis. Where facilities are available, rapid diagnosis can be made by electron microscopy (EM) of fecal material from cases with typical signs of disease. The virus also can be isolated in several feline and canine cell lines such as canine and feline kidney cells, but isolation is seldom used in practice since cell cultures are required and at least 1 week for results is required. Fecal hemagglutination-hemagglutination (HA-HI) tests have provided a simple and rapid method for detecting virus in fecal and tissue samples and are employed by several diagnostic laboratories, however the HA test is less sensitive than EM or enzyme-linked immunoassays (ELISA). In this work, the laying chickens are immunized with the canine parvovirus strain Cornell 780916-80 and the egg yolk antibody (IgY) isolated and characterized by indirect ELISA to detect canine parvovirus in feces. Salvador

Keywords: Canine parvovirus - Diagnosis - Egg yolk antibody- ELISA, indirect; Canine parvovirus - Diagnosis - HA-HI Submitted by ROBERTO PAULO CORREIA DE ARAÚJO (ppgorgsistem@ufba.br) on 2016-09-15T16:43:52Z No. of bitstreams: 1 4_v.7_3.pdf: 214631 bytes, checksum: c74d99fe9749dea1e8fb6759c7a3c70a (MD5) Made available in DSpace on 2016-09-15T16:43:52Z (GMT). No. of bitstreams: 1 4_v.7_3.pdf: 214631 bytes, checksum: c74d99fe9749dea1e8fb6759c7a3c70a (MD5) Previous issue date: 2008-09 Canine parvovirus (CPV), cause an intestinal disease characterized by bloody diarrhea, is often fatal in puppies. The virus is transmitted by contact with infected dogs or their feces. The virus is very stable in the environment and may survive for several months in contaminated areas. The CPV attacks the rapidly diving cells of the bone marrow and the small intestine. Several laboratory tests have been developed and are available for specific viral diagnosis. Where facilities are available, rapid diagnosis can be made by electron microscopy (EM) of fecal material from cases with typical signs of disease. The virus also can be isolated in several feline and canine cell lines such as canine and feline kidney cells, but isolation is seldom used in practice since cell cultures are required and at least 1 week for results is required. Fecal hemagglutination-hemagglutination (HA-HI) tests have provided a simple and rapid method for detecting virus in fecal and tissue samples and are employed by several diagnostic laboratories, however the HA test is less sensitive than EM or enzyme-linked immunoassays (ELISA). In this work, the laying chickens are immunized with the canine parvovirus strain Cornell 780916-80 and the egg yolk antibody (IgY) isolated and characterized by indirect ELISA to detect canine parvovirus in feces. Salvador