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Junfei Xu,1,2 Feiran Wang,2 Xi Wang,3 Zhixian He,2 Xinguo Zhu1 1Department of General Surgery, First Affiliated Hospital, Soochow University, Suzhou, People’s Republic of China; 2Department of General Surgery, Affiliated Hospital, Nantong University, Nantong, People’s Republic of China; 3Medical College of Nantong University, Nantong, People’s Republic of China Background/purpose: Given the emerging role of microRNA (miRNA) in cancer progression, we investigated the role and mechanism of miRNA-543 (miR-543) in gastric cancer (GC).Materials and methods: Real-time quantitative polymerase chain reaction was conducted to quantify the expression of miR-543. Luciferase reporter assay was used to confirm the association between speckle-type POZ protein (SPOP) and 3'-UTR. Moreover, the role of miR-543 and SPOP in GC was detected using transwell assays. In addition, we investigated the function of miR-543 in the epithelial–mesenchymal transition (EMT) progression.Results: miR-543 was upregulated in GC. We identified SPOP as a direct target of miR-543, revealing its expression to be inversely correlated with miR-543 expression in GC tissues. Moreover, restoration of SPOP could inhibit miR-543-induced GC cell migration and invasion, whereas downregulation of miR-543 inhibited cell migration and invasion, which was partly abrogated by SPOP knockdown. Furthermore, our data also showed that miR-543 induced EMT of GC cells.Conclusion: Our results demonstrated that miR-543 functions as a crucial oncogenic miRNA in GC. It exerts strong tumor-promoting effects through targeting SPOP in GC cell migration and invasion. Keywords: gastric cancer, miR-543, SPOP, EMT, invasion and migration

The crankshaft bearing in an internal combustion engine is a typical dynamically loaded bearing. In an engine, the crankshaft and bearing constitute an inseparable system. When operating, many mechanical actions in this system happen simultaneously and influence each other. This article presents a method to obtain the journal center trajectory that considers the interaction of the crankshaft and bearing. The dynamic equations of motion of the crankshaft and the Reynolds equations were solved simultaneously to determine (calculate) the load on the bearing, the dynamic response (journal center trajectory), and the tribological performance of the bearings. The numerical results show that the journal center trajectory of a crankshaft and tribological performance of bearings calculated by this method are remarkably different from those obtained by current methods.

Formulae of the transformation of Gaussian beams through conical diverging graded-index rods with a Kerr nonlinearity are derived from the variational method and the ABCD law. It is shown that ray paths and the effective rod length are strongly dependent on the beam power, which has no counterpart in the linear case and under the geometrical optics approximation.