1. Comparative cytotoxic effects of five commonly used triazole alcohol fungicides on human cells of different tissue types
- Author
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Xianfei Zhang, Xuefeng Liu, Hui Xiong, Jiuyong Xu, Qingchun Huang, Solange Muhayimana, and Yufan Xue
- Subjects
0301 basic medicine ,Cell Survival ,Apoptosis ,010501 environmental sciences ,01 natural sciences ,HeLa ,03 medical and health sciences ,Toxicity Tests ,Humans ,Cytotoxic T cell ,Viability assay ,Cytotoxicity ,0105 earth and related environmental sciences ,A549 cell ,biology ,Chemistry ,General Medicine ,Triazoles ,HCT116 Cells ,biology.organism_classification ,Apoptotic body ,Pollution ,Fungicides, Industrial ,030104 developmental biology ,Biochemistry ,A549 Cells ,K562 Cells ,Reactive Oxygen Species ,HeLa Cells ,Food Science ,K562 cells - Abstract
The widespread application of triazole fungicides makes people attach great concern over its adverse effects in mammalian. In this paper, cytotoxic effects of triazole alcohol fungicides (TAFs) were assessed on human HeLa, A549, HCT116 and K562 cells, and the potential mechanism of TAFs cytotoxicity was studied preliminarily. Results showed that TAFs had cytotoxicity on human cells with different level and cytotoxic selectivity. TAFs cytotoxicity was resonated with a typical hormetic biphasic dose action that produced a complex pattern of stimulatory or inhibitory effects on cell viability. Among the five TAFs, diniconazole revealed a widest range of cytotoxicity to inhibit the viability of the adherent and the suspension cells, causing HeLa cells shrinkage, A549 cells shrunken, and K562 cells collapse, and showed stronger cytotoxicity than hexaconazole. Moreover, the involvement of ROS generation in the cytotoxicity of TAFs on human cells was observed, and the apoptosis of HeLa cells and the formation of apoptotic body in K562 cells induced by diniconazole were characterized. The results indicated the cytotoxicity of TAFs with different structures on human cells was depended on their own property and cell specificity, K562 cells were the most susceptible to TAFs and diniconazole was the strongest toxic.
- Published
- 2020