1. Enhancement of DNA double-strand break induction and cell killing by K-shell absorption of phosphorus in human cell lines
- Author
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Masanori Tomita, Ritsuko Watanabe, K. Kobayashi, Munetoshi Maeda, Akinari Yokoya, and Noriko Usami
- Subjects
0301 basic medicine ,Programmed cell death ,Cell cycle checkpoint ,DNA damage ,DNA repair ,Apoptosis ,Electrons ,HeLa ,03 medical and health sciences ,0302 clinical medicine ,Nuclear magnetic resonance ,Cell Line, Tumor ,Humans ,Radiology, Nuclear Medicine and imaging ,Radiological and Ultrasound Technology ,biology ,Absorption, Radiation ,Dose-Response Relationship, Radiation ,Phosphorus ,Radiotherapy Dosage ,Neoplasms, Experimental ,Cell cycle ,biology.organism_classification ,Treatment Outcome ,030104 developmental biology ,Cell killing ,030220 oncology & carcinogenesis ,Biophysics ,DNA Damage - Abstract
Purpose: To investigate an enhancement of DNA double-strand break (DSB) induction and cell killing effect by K-shell ionization of phosphorus atoms and Auger electrons on human cell lines. Materials and methods: Induction of DSB, DNA damage responses, cell cycle distributions, and cell killing effects were investigated after exposures of the cells with monochromatic synchrotron radiation soft X-rays of 2153 and 2147 eV, which were the resonance peak and off peak, respectively, of the K-shell photoabsorption of phosphorus. Results: Higher biological effects in the cells irradiated with soft X-rays at 2153 eV than at 2147 eV were observed in (i) the efficiency of 53BP1/γ-H2AX co-localized foci formation per dose and residual number of foci, (ii) prolonged phosphorylation levels of DSB repair and/or cell cycle checkpoint related proteins and G2 arrest, (iii) the cell killing effects at the 10% survival level of normal human fibroblasts, HeLa cells, and human glioblastoma M059K cells (1.2–1.5 times higher) and that of human ataxia telangiectasia mutated (ATM)-defective cells and glioblastoma DNA-dependent protein kinase catalytic subunit (DNA-PKcs)-defective cells (1.2 times). Conclusion: The yield of DSB and partly less-reparable complex DNA damage induction in human cells was enhanced by K-shell photoabsorption of phosphorus and low-energy Auger electrons.
- Published
- 2016