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In order to replace the antibiotic treatment for control of Vibrio harveyi, a causal agent of luminous disease in Black tiger shrimp, anti-V. harveyi IgY was produced and showed its potential in our preliminary study. However, for further use as feed additive, the IgY stability should be evaluated. The titre of specific IgY was enhanced with an immunostimulant, C-phosphate guanosine oligodeoxynucleotide (CpG-ODN). IgY was stable at natural pH while the activity was decreased below pH 4 or above pH 10. The supplementation of 30% sorbitol significantly enhanced the IgY stability at tested temperatures in excess of 70°C. The activity of IgY remained at 9 and 94% after 4 h incubation in simulated gastric and intestinal fluids, respectively. A storage temperature at −20°C was found to be the best condition. IgY at the concentrations of 1, 5 and 10 mg/ml efficiently inhibited V. harveyi in vitro within 24 h after exposure.

Aflatoxins (AFs) and aflatoxin B1 (AFB1) intake through consumption of peanuts and peanut products were estimated from 60 samples. The samples were collected from 2 retail and 2 wholesale markets in Bangkok during November 2013–January 2014. The results showed that 80% of raw peanut samples were contaminated with AFs while 100% of roasted and ground peanuts samples were contaminated. The highest concentration of AFs was found in ground peanuts at 362.48 ng g−1 resulting in the highest mean concentration (68.22 ng g−1). According to a food consumption database, the average intake of AFs was estimated at 0.49, 0.40 and 2.13 ng/kg bw/day for raw, roasted and ground peanuts, respectively. The potential risk for cancer was estimated at 0.01–0.12 cancer/year/100,000 persons. The results suggest that the current situation of aflatoxins contamination in peanuts and peanut products (especially in ground peanuts) has an adverse effect on the health of the Thai population.