1. Biocidal action of ozone against planktonic and sessilePseudomonas fluorescens
- Author
-
Hector A. Videla, Marisa Viera, Patricia Sandra Guiamet, and Mónica Alicia Fernández Lorenzo de Mele
- Subjects
Biocide ,Ozone ,fungi ,Biofilm ,Biofilm matrix ,Pseudomonas fluorescens ,Penetration (firestop) ,Aquatic Science ,Biology ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Microbiology ,chemistry.chemical_compound ,Extracellular polymeric substance ,chemistry ,Environmental chemistry ,Bacteria ,Water Science and Technology - Abstract
The efficacy of ozone biocidal action against planktonic and sessile Pseudomonas fluorescens was studied using different contact times and biocide concentrations. Bacterial biofilms were formed on different metallic substrata frequently used as structural materials in industrial installations. The results showed that the biocidal action of ozone against planktonic cells can be represented by a dose‐response relationship in which the contact time factor was more significant than the concentration. The efficacy of ozone against sessile bacteria was lower than that found for planktonic cells. Turbulent flow significantly improved the inactivation rate, suggesting that a diffusional impediment was involved in the mass transfer process. However, diffusional resistance alone cannot explain the observed decrease in biocidal efficacy. Ozone penetration into the biofilm appeared to be a function of simultaneous diffusion and a reaction of the biocide in the biofilm matrix; the latter may be the result of microscal...
- Published
- 1999