1. Analysis of Total Platinum Derived from Cisplatin in Tissue
- Author
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Michael L. Denniston, Larry A. Sternson, and A.J. Repta
- Subjects
Cisplatin ,Residue (complex analysis) ,Chromatography ,Chemistry ,Biochemistry (medical) ,Clinical Biochemistry ,Analytical chemistry ,chemistry.chemical_element ,Combustion ,Biochemistry ,Analytical Chemistry ,law.invention ,chemistry.chemical_compound ,law ,Electrochemistry ,medicine ,Aqua regia ,Platinum ,Atomic absorption spectroscopy ,Spectroscopy ,medicine.drug - Abstract
A method is described for determining total platinum in tissues of animals receiving cisplatin. The method is based on total combustion of the tissue, leaving a platinum residue devoid of organic material that can be dissolved in aqua regia and analyzed by carbon-rod furnace atomic absorption spectrophotometry. Combustion eliminates matrix effects in final read-out, providing a means for precisely and reproducibly analyzing a variety of tissues. Platinum levels of 200 ng/0.5 g of sample can readily be analyzed with recoveries from all tissues approaching 100%. The validity of the method is demonstrated by giving rats intravenous doses of cisplatin and subsequently measuring tissue levels of platinum.
- Published
- 1981
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