1. Endcaps for Stabilizing Short DNA Duplexes
- Author
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Alan M. Friedman, Ganesan Balasundarum, Xiaolin Zu, Pei-Sze Ng, Maneesh Pingle, and Donald E. Bergstrom
- Subjects
Models, Molecular ,chemistry.chemical_classification ,Dna duplex ,Base Sequence ,Stereochemistry ,DNA ,General Medicine ,Nucleic Acid Denaturation ,Biochemistry ,chemistry.chemical_compound ,Terthiophene ,Drug Stability ,chemistry ,Covalent bond ,Amide ,Nucleic acid ,Genetics ,Nucleic Acid Conformation ,Thermodynamics ,Molecular Medicine ,Nucleotide ,Histone octamer - Abstract
The syntheses of endcaps for covalently linking the 3′ and 5′ hydroxyl groups of blunt end double-stranded DNA are described. Endcap diols were converted into DMTr protected phosphoramidites and incorporated between nucleotides 4 and 5 of a self-complementary octamer. The stabilizing effect of the endcaps on duplex DNA was determined by Tm experiments on the self-complementary octamer.
- Published
- 2003
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