1. Low dose of uranium induces multigenerational epigenetic effects in rat kidney
- Author
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Christelle Elie, Philippe Lestaevel, Céline Gloaguen, Dimitri Kereselidze, Stéphane Grison, Ghada Elmhiri, Audrey Legendre, Line Manens, Mohamed Amine Benadjaoud, Karine Tack, Maâmar Souidi, Jean-Marc A. Lobaccaro, PSE-SANTE/SESANE/LRTOX, Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PSE-SANTE/SERAMED, Génétique, Reproduction et Développement (GReD), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de radiotoxicologie et radiobiologie expérimentale (IRSN/PSE-SANTE/SESANE/LRTOX), Service de recherche sur les effets biologiques et Sanitaires des rayonnements ionisants (IRSN/PSE-SANTE/SESANE), Institut de Radioprotection et de Sûreté Nucléaire (IRSN)-Institut de Radioprotection et de Sûreté Nucléaire (IRSN), Laboratoire de Radiobiologie des expositions médicales (IRSN/PSE-SANTE/SERAMED/LRMed), Service de recherche en radiobiologie et en médecine régénérative (IRSN/PSE-SANTE/SERAMED), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS)
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Male ,inorganic chemicals ,0301 basic medicine ,Chronic exposure ,medicine.medical_specialty ,[SDV]Life Sciences [q-bio] ,Rat kidney ,chemistry.chemical_element ,Biology ,Kidney ,complex mixtures ,Epigenesis, Genetic ,Rats, Sprague-Dawley ,03 medical and health sciences ,Pregnancy ,Internal medicine ,medicine ,Animals ,Radiology, Nuclear Medicine and imaging ,Epigenetics ,Sexual difference ,Radiological and Ultrasound Technology ,Body Weight ,Low dose ,technology, industry, and agriculture ,Dose-Response Relationship, Radiation ,DNA Methylation ,Uranium ,Rats ,3. Good health ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,chemistry ,[SDV.TOX]Life Sciences [q-bio]/Toxicology ,DNA methylation ,Female - Abstract
International audience; Purpose A protocol of chronic exposure to low dose of uranium was established in order to distinguish the sexual differences and the developmental process that are critical windows for epigenetic effects over generations. Methods Both male and female rats were contaminated through their drinking water with a non-toxic solution of uranyl nitrate for 9 months. The exposed generation (F0) and the following two generations (F1 and F2) were examined. Clinical monitoring, global DNA methylation profile and DNA methyltransferases (DNMTs) gene expression were analyzed in kidneys. Results While the body weight of F1 males increased, a small decrease in kidney and body weight was observed in F2 males. In addition, global DNA hypermethylation profile in kidney cells was observed in F1 and F2 males. qPCR results reveal a significant increase of methyltransferase genes expression (DNMT1 and DNMT3a) for F2 females. Conclusions In the field of public health policy and to raise attention to generational effects for the risk assessment of the environmental exposures, low doses of uranium do not imply clinical effects on adult exposed rats. However, our results confirm the importance of the developmental windows’ sensitivity in addition to the sexual dimorphisms of the offspring. © 2018, Copyright © 2018 Taylor and Francis Group LLC.
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- 2018
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