1. Synthesis, characterization, and DNA binding studies of the hematoxylin-Zn(II) complex
- Author
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Kang Xue, Chao You, Xiaoli Xiong, Jian-Hang Huang, and Chun-Ling Wang
- Subjects
Scatchard plot ,010405 organic chemistry ,Stereochemistry ,Intercalation (chemistry) ,Acridine orange ,Herring sperm DNA ,010402 general chemistry ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,Viscosity measurement ,Inorganic Chemistry ,chemistry.chemical_compound ,Crystallography ,chemistry ,Ionic strength ,Physical and Theoretical Chemistry ,DNA - Abstract
The binding mechanism of Hematoxylin-Zn(II) complex with Herring sperm DNA has been investigated using UV–vis and fluorescence spectroscopies and viscosity measurement. With the increase in the concentration of complex, the viscosity of DNA decreased, and the binding mode is more detailed: there is partially intercalate binding. Furthermore, besides the intercalate binding, the existence of groove binding was confirmed through the ionic strength experiment in Scatchard analysis. Thermodynamic studies provided some thermodynamic parameters: ΔrHmΘ = 1.43 × 105 J mol−1, ΔrSmΘ = 576.14 J mol−1, ΔrGmΘ303K = −3.16 × 104 J mol−1, they illustrated that the binding mechanism between HE-Zn(II) complex and hsDNA was driven mainly by entropy.
- Published
- 2016
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