1. Signal-on fluorescent sensor based on N-CQDs for the detection of glutathione in human serum and pharmaceutic preparation
- Author
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Kangying Wang, Linghong Pan, Liumin Yu, Haijian Tu, Qihong Cai, and Chengfei Zhao
- Subjects
Nitrogen ,Ethylenediamine ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Fluorescence ,chemistry.chemical_compound ,Limit of Detection ,Quantum Dots ,Humans ,chemistry.chemical_classification ,Detection limit ,Chromatography ,General Medicine ,Glutathione ,021001 nanoscience & nanotechnology ,Ascorbic acid ,Signal on ,Carbon ,0104 chemical sciences ,Amino acid ,Spectrometry, Fluorescence ,chemistry ,0210 nano-technology ,Citric acid ,Tablets ,Biotechnology - Abstract
Nitrogen-doped carbon quantum dots (N-CQDs) with citric acid and ethylenediamine as raw materials were synthesized by an efficient one-step strategy. The N-CQDs showed a special property that the fluorescence was quenched by Fe3+. The quenched fluorescence of N-CQDs could be recovered by glutathione (GSH). Therefore, a "signal-on" fluorescent sensor was developed to detect GSH. The fluorescent sensor could favorably avoid the interference of ascorbic acid, dopamine, glucose, oxidized glutathione, and other amino acids in the detecting process of GSH. The proposed sensor showed a great feature that GSH can be accurately detected in the range from 0.001 to 0.1 mol/L and can be applied to detect GSH in the human serum. Therefore, the proposed method has a promising application for monitoring the blood drug concentration of GSH in clinical studies.
- Published
- 2017
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