1. Urinary exosomal expression of long non-coding RNAs as diagnostic marker in bladder cancer
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Maryam Eghbali, Leila Farhady Tooli, Elahe Motevaseli, Mandana Afsharpad, Leila Nekoohesh, Vahid Kholghi Oskooei, Soudeh Ghafouri-Fard, Seyed Javad Mowla, Fatemeh Yazarlou, and Mohammad Hossein Modarressi
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0301 basic medicine ,Urinary system ,Cell ,urologic and male genital diseases ,Exosome ,03 medical and health sciences ,lncRNA ,0302 clinical medicine ,medicine ,exosome ,Original Research ,MALAT1 ,Bladder cancer ,business.industry ,Diagnostic marker ,medicine.disease ,humanities ,female genital diseases and pregnancy complications ,Microvesicles ,030104 developmental biology ,Transitional cell carcinoma ,medicine.anatomical_structure ,Oncology ,Cancer Management and Research ,030220 oncology & carcinogenesis ,Cancer research ,bladder cancer ,business - Abstract
Fatemeh Yazarlou,1 Mohammad Hossein Modarressi,1 Seyed Javad Mowla,2 Vahid Kholghi Oskooei,3 Elahe Motevaseli,4 Leila Farhady Tooli,5 Leila Nekoohesh,6 Maryam Eghbali,1 Soudeh Ghafouri-Fard,3 Mandana Afsharpad7 1Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; 2Faculty of Biological Sciences, Department of Genetics, Tarbiat Modares University, Tehran, Iran; 3Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 4Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; 5Department of Microbiology, School of Biology, College of Science, Tehran University, Tehran, Iran; 6Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; 7Cancer Control Research Center, Cancer Control Foundation, Iran University of Medical Sciences, Tehran, Iran Background: Long non-coding RNAs (lncRNAs) and exosomes have been regarded as components of cell signal transmission that modulate indigenous cellular microenvironments. Exosomes also participate in relocation of functional lncRNAs between cells. Methods: In the present study, we evaluated expression of LINC00355, LINC00958, UCA1-201, UCA1-203, and MALAT1 lncRNAs in urinary exosomes isolated from transitional cell carcinoma (TCC) of bladder, non-malignant urinary disorders, and normal subjects. Results: LINC00355, UCA1-203, and MALAT1 expression was significantly higher in TCC patients compared to controls (non-malignant or normal samples). However, UCA1-201 expression was significantly decreased in TCC patients compared with controls. LINC00355 and MALAT1 expression was significantly lower in cigarette smokers and opium-addicted TCC patients, respectively. On the other hand, LINC00355 expression tended to be higher in opium-addicted TCC patients. The proposed panel of lncRNAs (composed of UCA1-201, UCA1-203, MALAT1, and LINC00355) had 92% sensitivity and 91.7% specificity for diagnosis of bladder cancer from normal samples. Conclusion: Transcript levels of lncRNAs in urinary exosomes are potential diagnostic biomarkers in bladder cancer. Keywords: lncRNA, exosome, bladder cancer
- Published
- 2018
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