1. Quantitation of Amiodarone and Desethylamiodarone from Blood Serum and Myocardium Using Reverse Phase HPLC
- Author
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Cyril Y. Leung, James E. Lowe, Robin G. Cummings, D. James Schumacher, J. Alan Menius, and Emily A. Hull-Ryde
- Subjects
Chromatography ,Myocardial tissue ,Chemistry ,Metabolite ,Reversed-phase chromatography ,Amiodarone ,Lower limit ,chemistry.chemical_compound ,Therapeutic index ,Blood serum ,medicine ,Molecular Medicine ,Solid phase extraction ,medicine.drug - Abstract
A new method for quantitating amiodarone and its metabolite desethylamiodarone from serum and myocardial tissue is described. Serum and tissue components are initially removed before analysis with C18 solid phase extraction columns. Quantitation is then achieved using a simple step gradient and a C18 reverse phase column. Percent recovery of amiodarone is greater than 90 percent in both serum and myocardial tissue and is linear throughout the therapeutic range with a lower limit sensitivity of .04 μg/ml. No commonly used cardiovascular drugs were found to interfere with the assay.
- Published
- 1987
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