1. Research on paraffin removal and prevention by Bacillus spp. in high-salinity reservoirs
- Author
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Y. W. Gao, Xiaonan Liu, Y. J. Luo, Y. Z. Wei, Y. Zhuang, Te Sha, Wang Jiexiang, Q. S. Xu, Zhen Zhang, Shanshan Sun, C. J. Wang, Z. Jin, and Chang Liu
- Subjects
Chromatography ,Chemistry ,General Chemical Engineering ,Energy Engineering and Power Technology ,02 engineering and technology ,General Chemistry ,Bacillus sp ,010501 environmental sciences ,Biodegradation ,Geotechnical Engineering and Engineering Geology ,Crude oil ,01 natural sciences ,Salinity ,Fuel Technology ,020401 chemical engineering ,0204 chemical engineering ,Gas chromatography–mass spectrometry ,Deposition (chemistry) ,0105 earth and related environmental sciences - Abstract
Problems in paraffin deposition occur frequently in oil exploitation. Considering the problem that some paraffin removal and prevention strains cannot grow in high-salinity reservoirs, we domesticated four salt-tolerant laboratory bacterial strains. Crude oil emulsification effect, paraffin removal rate, and paraffin prevention rate of the strains were evaluated. KB and JH-A strains exhibited good paraffin removal and prevention effects, with paraffin removal rates of 51.82% and 81.56%; and 51.82% and 80.90%, respectively. Gas chromatography mass spectrometry analysis of paraffin components before and after biodegradation was performed on KB and JH-A strains. Results showed that relative contents of n-alkanes between C15–C23 and C27–C30 evidently decreased, indicating their good biodegradation. This technique provides a new method for practical application of paraffin removal and prevention in high-salinity reservoirs.
- Published
- 2018
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