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Multidrug resistance is one of the key barriers suppressing the effectiveness of drug therapies of malignant tumors. Here, we report a study on the effect of a mix of natural extracts (MIX2) prepared from fresh fruits of Prunus spinosa, Crataegus monogyna, Sorbus aucuparia, and Euonymus europaeus on the classic hallmarks of cancer cells and the expression of multidrug resistance proteins. In the studies, HeLa and T98G cell lines, and classic methods of molecular biology, including RT-qPCR, Western blot, flow cytometry, and confocal imaging, were used. Additionally, migration, adhesion, and proliferation assays were performed. The obtained results indicate that the MIX2 cocktail presents strong anti-cancer properties. MIX2 is not toxic, but at the same time significantly alters the migration, proliferation, and adhesion of tumor cells. Furthermore, it was found that cells exposed to the mixture presented a significantly reduced expression level of genes associated with MDR, including ABCB1, which encodes for glycoprotein P. In vitro data showed that MIX2 effectively sensitizes tumor cells to doxorubicin. We postulate that modulation of the multidrug resistance phenotype of tumors with the use of MIX2 may be considered as a safe and applicable tool in sustaining drug delivery therapies of malignancies.

The objective of the study was to compare the horse's body parts in position at the first approach stride, with the size of the jump. The size of the jump was accepted as the measure of the horse's performance. The experiment included the filming of 540 young warmblood stallions free jumping a 120 cm high oxer. Ten traits assumed to be connected with the size of the jump were measured by digital image analysis. Pearson's correlations between the parameters were calculated. Our results demonstrated the four traits that are most important in judgement of the young horse's jumping ability at the approach stride 1. The low angle of bending at poll positively influences the size of the jump. Angles of the positions of neck and trailing hindlimb cannon have a positive effect only up to 100° and up to 40°, respectively. The poll–obstacle distance should not exceed 120 cm.

The objective of the study was to devise methods that assess judging of horses in the dressage discipline. Classes run at the Olympic Games were analyzed. The data contained 30,055 marks awarded for single movements. The accuracy of the judging was measured with the Index of Disagreement that compares the horse ranking in a movement determined by a judge, to the ranking resulted from the marks awarded by other members of the jury. The authors proposed Horse Mean Placing Standard Deviation to test the consistency in judging particular horses. The study showed that the horse's scores were not sufficiently reliable and should be processed by checking the quality of judging before using the results both in breeding or sports and in further scientific analyses.