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In the present study, the possible role of ellagic acid (EA) on antioxidant potential of Epilobium hirsutum (EH) in rat liver was investigated. Wistar rats were intraperitoneally treated with 37.5 mg/kg of EH and 10 mg/kg of EA for 9 days. Effects of EH and EA on antioxidant [glutathione peroxidase (GPx) and superoxide dismutases (SOD)] and Phase II [NADPH quinone oxidoreductase 1 (NQO1) and glutathione S-transferases (GSTs)] enzyme activities, as well as protein and mRNA expressions of those, were investigated. Polyphenolic content of EH was determined by LC-MS/MS analysis. EH and EA injection to rats resulted in a significant increase of NQO1 (3.6-fold and 4.7-fold), GPx (1.45-fold), and SOD (1.34-fold and 1.27-fold) enzyme activities, whereas total GST (46% and 57%) and its isoforms,and GST mu (57% and 72%), and GST theta (60% and 68%) activities were significantly decreased. Western-blot and qRT-PCR analysis showed that NQO1 and GPx protein and mRNA expressions were increased significantly (P < 0.0001), whereas GST mu and GST theta were significantly decreased (P < 0.0001). © 2016 Taylor & Francis Group, LLC.

In this study, pollution in Curuksu Stream of Menderes River has been followed by measuring the chemical and biological parameters on five predetermined stations for about 20 mon. Our previous studies have shown that Limnodrilus profundicola, Eristalis sp. and Chironomus thummi taxa are valuable bioindicators for the Curuksu stream and Limnodrilus profundicola Metallo-Thionein (MT) and EthoxyResorufi n-O-deEthylase (EROD) levels are valuable and useful biomarkers for biomonitoring heavy metals and PAHs pollutions, respectively. Cu-, Cr-, Cd- and Pb-type pollution was detected in all sediment samples taken from all of the stations, including reference station. Pollution source of these heavy metals are considered to be industrial wastewater, atmosphere and soil. Similarly, PAHs level was found to be considerably higher for Curuksu and Guzelkoy stations than other stations. These heavy metal- and PAHs-pollution were also confirmed with elevated MT and EROD levels measured with Limnodrilus profundicola sampl...