12 results on '"Ruozi, Barbara"'
Search Results
2. Sialic acid as a potential approach for the protection and targeting of nanocarriers
- Author
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Bondioli, Lucia, primary, Ruozi, Barbara, additional, Belletti, Daniela, additional, Forni, Flavio, additional, Vandelli, Maria Angela, additional, and Tosi, Giovanni, additional
- Published
- 2011
- Full Text
- View/download PDF
3. Polymeric nanoparticles for the drug delivery to the central nervous system
- Author
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Tosi, Giovanni, primary, Costantino, Luca, additional, Ruozi, Barbara, additional, Forni, Flavio, additional, and Vandelli, Maria Angela, additional
- Published
- 2008
- Full Text
- View/download PDF
4. Antioxidant activity and photostability assessment of trans-resveratrol acrylate microspheres.
- Author
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Pignatello R, Pecora TMG, Cutuli GG, Catalfo A, De Guidi G, Ruozi B, Tosi G, Cianciolo S, and Musumeci T
- Subjects
- Acrylic Resins chemistry, Calorimetry, Differential Scanning methods, Drug Compounding methods, Microspheres, Polymethacrylic Acids chemistry, Solubility, Spectroscopy, Fourier Transform Infrared methods, Acrylates chemistry, Antioxidants chemistry, Polymers chemistry, Resveratrol chemistry
- Abstract
Trans-resveratrol (RSV) was microencapsulated in Eudragit
® RS100 and RL100 resin blends. Lyophilized microspheres were characterized in the solid state for their micromeritic properties and drug loading. FT-IR, PXRD, and DSC analyzes suggested that RSV formed an intimate microcrystalline dispersion within the polymer network, also confirmed by SEM analysis. This produced a reduced degradation of RSV after storage at 40 °C, compared to the neat drug, and a protection of the drug from UV light-induced trans-cis isomerization (60% intact drug was found after 60 s irradiation at 350 nm, compared to 37% for the pure drug). Solubility and in vitro dissolution studies indicated that microencapsulation did not improve the dissolution pattern of RSV in simulated gastric and intestinal aqueous fluids. Evaluation of the in vitro antioxidant activity showed that, compared to the neat drug in aqueous solution, RSV loaded in the microspheres retained for a longer time, up to 22 days of incubation, the initial ORAC capacity. The present study thus demonstrated that Eudragit® Retard resins can be used to easily produce micro-sized solid dispersions with RSV, for potential oral administration, contributing to ameliorate the physico-chemical stability and antioxidant activity of this compound.- Published
- 2019
- Full Text
- View/download PDF
5. Protein cage nanostructure as drug delivery system: magnifying glass on apoferritin.
- Author
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Belletti D, Pederzoli F, Forni F, Vandelli MA, Tosi G, and Ruozi B
- Subjects
- Animals, Antineoplastic Agents chemistry, Drug Liberation, Ferritins chemistry, Ferritins pharmacokinetics, Humans, Nanomedicine, Nanostructures chemistry, Neoplasms metabolism, Antineoplastic Agents administration & dosage, Drug Delivery Systems, Ferritins administration & dosage, Nanostructures administration & dosage
- Abstract
Introduction: New frontiers in nanomedicine are moving towards the research of new biomaterials. Apoferritin (APO), is a uniform regular self-assemblies nano-sized protein with excellent biocompatibility and a unique structure that affords it the ability to stabilize small active molecules in its inner core. Areas covered: APO can be loaded by applying a passive process (mainly used for ions and metals) or by a unique formulative approach based on disassemby/reassembly process. In this article, we aim to organize the experimental evidence provided by a number of studies on the loading, release and targeting. Attention is initially focused on the most investigated antineoplastic drug and contrast agents up to the most recent application in gene therapy. Expert opinion: Various preclinical studies have demonstrated that APO improved the potency and selectivity of some chemotherapeutics. However, in order to translate the use of APO into therapy, some issues must be solved, especially regarding the reproducibility of the loading protocol used, the optimization of nanocarrier characterization, detailed understanding of the final structure of loaded APO, and the real mechanism and timing of drug release.
- Published
- 2017
- Full Text
- View/download PDF
6. Apoferritin nanocage as drug reservoir: is it a reliable drug delivery system?
- Author
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Tosi G, Belletti D, Pederzoli F, and Ruozi B
- Published
- 2016
- Full Text
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7. Functionalization of liposomes: microscopical methods for preformulative screening.
- Author
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Belletti D, Vandelli MA, Tonelli M, Zapparoli M, Forni F, Tosi G, and Ruozi B
- Subjects
- Microscopy, Atomic Force, Particle Size, Surface Properties, Liposomes chemistry
- Abstract
The development of smart delivery systems able to deliver and target a drug to the site of action is one of the major challenges in the field of pharmaceutical technology. The surface modification of nanocarriers, such as liposomes, is widely investigated either for increasing the blood circulation time (by pegylation) or for interacting with specific tissues or cells (by conjugation of a selective ligand as a monoclonal antibody, mAb). Microscopical analysis thereby is a useful approach to evaluate the morphology and the size owing to resolution and versatility in defining either surface modification or the architecture and the internal structure of liposomes. This contribution aims to connect the outputs obtained by transmission electron (TEM) and atomic force (AFM) microscopical techniques for identifying the modifications on the liposomal surface. To reach this objective, we prepared liposomes applying two different pegylation technologies and further modifying the surface by mAb conjugation. This work demonstrates the feasibility to apply the combined approach (TEM and AFM analysis) in the evaluation of the efficacy of a surface engineering process.
- Published
- 2015
- Full Text
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8. AFM phase imaging of soft-hydrated samples: a versatile tool to complete the chemical-physical study of liposomes.
- Author
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Ruozi B, Tosi G, Tonelli M, Bondioli L, Mucci A, Forni F, and Vandelli MA
- Subjects
- Magnetic Resonance Spectroscopy methods, Surface Properties, Liposomes chemistry, Microscopy, Atomic Force methods, Polyethylene Glycols chemistry
- Abstract
Despite of the several approaches applied to the physicochemical characterization of liposomes, few techniques are really useful to obtain information about the surface properties of these colloidal drug-delivery systems. In this paper, we demonstrate a possible new application of tapping mode atomic force microscopy (AFM) to discriminate between conventional and pegylated liposomes. We showed that the differences on liposomal surface properties revealed by the phase images AFM approach well correlate with the data obtained using classical methods, such as light scattering, hydrodynamic, and nuclear magnetic resonance analysis.
- Published
- 2009
- Full Text
- View/download PDF
9. Flow cytometry and live confocal analysis for the evaluation of the uptake and intracellular distribution of FITC-ODN into HaCaT cells.
- Author
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Ruozi B, Montanari M, Vighi E, Tosi G, Tombesi A, Battini R, Restani C, Leo E, Forni F, and Vandelli MA
- Subjects
- Animals, Drug Carriers chemistry, Drug Carriers metabolism, Humans, Liposomes chemistry, Liposomes metabolism, Microscopy, Atomic Force, Microscopy, Electron, Transmission, Cell Line, Tumor metabolism, Flow Cytometry methods, Fluorescein chemistry, Fluorescein metabolism, Microscopy, Confocal methods, Oligodeoxyribonucleotides chemistry, Oligodeoxyribonucleotides metabolism
- Abstract
In this study, the mechanism of the internalization and the cellular distribution of 59 fluorescein conjugated PS-ODN (FITC-ODN) after transfection with different mixed lipidic vesicles/oligo complexes (lipoplexes) have been investigated. Mixed lipidic vesicles were prepared with one of the most used cationic lipid (DOTAP) and different amounts of a cholic acid (UDCA) to release the oligo into HaCaT cells. Using flow cytometry, the cellular uptake of the oligo was studied with and without different inhibitors able to block selectively the different pathways involved in the internalization mechanism. The intracellular distribution of the oligo was analyzed by confocal laser scanning microscopy (CLSM), treating the cells with the lipoplexes and directly observing without any fixing procedure. To better carry out the colocalization studies, fluorescent-labeled markers, specific for the different cellular compartments, were coincubated with 59 fluorescein-conjugated 29-mer phosphorotioate oligonucleotide (FITC-ODN). The different lipidic vesicles affect the internalization mechanism of FITC-ODN. After using the inhibitors, the uptake of complexes involved a different internalization mechanism. The live CLSM analysis demonstrated that, after 1 hour from the complex incubation, the oligo was transferred into cells and localized into the endosomes; after 24 hours, the oligo was intracellularly localized close to the nuclear structure in a punctuate pattern. However, the results from fusion experiments showed also a binding of a quite low amount of oligo with the cell membranes.
- Published
- 2009
- Full Text
- View/download PDF
10. Liposome-oligonucleotides interaction for in vitro uptake by COS I and HaCaT cells.
- Author
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Ruozi B, Battini R, Tosi G, Forni F, and Vandelli MA
- Subjects
- Animals, Antigens, Polyomavirus Transforming genetics, COS Cells, Cell Line, Cell Transformation, Neoplastic drug effects, Chlorocebus aethiops, Chromatography, High Pressure Liquid, Drug Carriers, Drug Compounding, Drug Delivery Systems, Electrophoresis, Polyacrylamide Gel, Flow Cytometry, Gene Transfer Techniques, Humans, Keratinocytes drug effects, Microscopy, Atomic Force, Microscopy, Fluorescence, Particle Size, Transfection, Liposomes, Oligonucleotides administration & dosage, Oligonucleotides pharmacokinetics
- Abstract
Liposomes are considered very promising delivery systems for antisense therapeutic approach, offering drug protection and facilitating oligonucleotide cell internalization. The present study was aimed to investigate the influence of phospholipid composition of the liposomal systems both on the encapsulation and on the oligonucleotide carrier capacity in vitro. Liposomes composed of neutral (phosphatidylcholine, cholesterol and dioleoylphosphatidylethanolamine) and/or cationic lipids (N-(1-(2,3-dioleoyloxy)propyl)-N,N,N-trimethylammonium chloride salt, DOTAP) with different molar ratios were complexed with 5' fluorescein conjugated 29-mer phosphorothioate oligonucleotide (PS-ODN). The interaction was evaluated using atomic force microscopy (AFM), gel electrophoresis and HPLC analysis. Cytofluorimetric analysis and fluorescence microscopy were applied to evaluate the uptake and intracellular distribution of fluorescently labelled PS-ODN after transfection in two cell lines, COS I (fibroblast cell) and HaCaT (immortalized keratinocyte cell). The AFM studies reveal that the liposome/PS-ODN interaction leads the formation of a new irregular structure that completely hides the PS-ODN. Gel electrophoresis experiments and HPLC analysis have clearly demonstrated that also neutral liposomes are able to keep a little amount of PS-ODN but without strain to the complexation; the interaction was weak and rapidly destabilized when the complex was added to the cells. Transfection experiments performed with different incubation times show that DOTAP liposomes increase the rate of cellular uptake of PS-ODN and seem to influence its intracellular distribution in COS I cells where the oligonucleotide looks localized in nucleoli. Similar behaviour, at a lesser extent, is exhibited in HaCaT cells.
- Published
- 2005
- Full Text
- View/download PDF
11. Ketorolac tromethamine liposomes: encapsulation and release studies.
- Author
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Ruozi B, Tosi G, Forni F, and Angela Vandelli M
- Subjects
- Cyclooxygenase Inhibitors chemistry, Drug Compounding, Humans, Microscopy, Atomic Force, Microscopy, Electron, Transmission, Particle Size, Anti-Inflammatory Agents, Non-Steroidal chemistry, Ketorolac Tromethamine chemistry, Liposomes chemistry
- Abstract
Liposomes loaded with ketorolac tromethamine salt were prepared by using a thin layer evaporation method. The physical properties of liposomes were studied by using atomic force microscopy (AFM) and transmission electron microscopy (TEM). The relationship between lipid composition, encapsulation efficiency, vesicle size, and the release of ketorolac tromethamine-loaded liposomes was studied. The drug content was found to be dependent on the lipidic composition used in the preparations and, in particular, vesicles containing both cationic lipids (dimethyldioctadecylammonium bromide and N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride), and phosphatidylcholine had a higher entrapped efficiency than liposomes with phosphatidylcholine alone or in the presence of cholesterol. Finally, the cationic liposomes appear to be useful as carriers for ketorolac tromethamine to control its in vitro release.
- Published
- 2005
- Full Text
- View/download PDF
12. Cationic liposomes for gene transfection.
- Author
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Ruozi B, Forni F, Battini R, and Vandelli MA
- Subjects
- Animals, Cations, Cell Line, Chlorocebus aethiops, Cytomegalovirus genetics, DNA administration & dosage, Drug Carriers, Electrophoresis, Agar Gel, Electrophoresis, Polyacrylamide Gel, Humans, Indicators and Reagents, Liposomes chemical synthesis, Mice, Microscopy, Atomic Force, Particle Size, Plasmids genetics, Liposomes chemistry, Transfection methods
- Abstract
Cationic liposomes spontaneously interact with the negatively charged DNA to form a stable complex that promotes the gene transfer to cells. The mode of formation and the size of cationic liposomes/DNA complexes were investigated using the atomic force microscopy (AFM). Also the most important physical-chemical factors involved in cationic liposome-mediated gene transfection, e.g. size and lipidic composition, were evaluated through the transfection of complexes with different liposomes/DNA molar ratio into three types of cultured cells. Cationic liposomes, composed of a neutral lipid (phosphatidilcoline), a cationic lipid dimethyldioctadecylammonium bromide (DDAB), a co-lipid 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE) and a phospholipid derivative of polyethylene glycol (DSPE-mPEG) at different molar ratio, were mixed with a plasmid pCMVbeta to form liposomes/DNA complexes. We have demonstrated that the complexes were made by complicated structures in which the liposomes tend to aggregate and the DNA is surrounded by lipidic material. In vitro transfection efficiency by liposomes/plasmid pCMVbeta complexes was found to depend on the kind of lipid associated in the liposomes and the liposomes/DNA mixing ratio. The importance of associating DOPE in cationic liposomes was confirmed; this co-lipid is able to improve the ability of cationic liposomes to transfect cells but in addition, the AFM images and the EtBr fluorescence experiments have suggested that this lipid can also play an important role to facilitate the formation of stable liposomes, which efficaciously protect the DNA by nuclease digestion.
- Published
- 2003
- Full Text
- View/download PDF
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