1. Dehydroepiandrosterone increased oxidative stress in a human cell line during differentiation.
- Author
-
Izumo K, Horiuchi M, Komatsu M, Aoyama K, Bandow K, Matsuguchi T, Takeuchi M, and Takeuchi T
- Subjects
- Cell Differentiation drug effects, Cell Differentiation physiology, Cell Growth Processes drug effects, Cell Growth Processes physiology, Cell Survival drug effects, Cell Survival physiology, Dimethyl Sulfoxide pharmacology, Glucosephosphate Dehydrogenase biosynthesis, Glucosephosphate Dehydrogenase genetics, Glucosephosphate Dehydrogenase metabolism, HL-60 Cells, Humans, NADPH Oxidases metabolism, NADPH Oxidases pharmacology, RNA, Messenger genetics, RNA, Messenger metabolism, Reactive Oxygen Species metabolism, Dehydroepiandrosterone pharmacology, Glucosephosphate Dehydrogenase antagonists & inhibitors, Oxidative Stress drug effects
- Abstract
Dehydroepiandrosterone (DHEA), a reversible inhibitor of glucose-6-phosphate dehydrogenase (G6PD), is increasingly taken as an antioxidative and anti-ageing supplement. This study investigated the effects of DHEA on the expression of G6PD and on the state of oxidative stress in a human promyelocytic leukaemia cell line, HL60, during the differentiation to neutrophil-like cell. This study differentiated HL60 with dimethyl sulfoxide (DMSO) in the presence (DMSO-HL60/DHEA) or absence (DMSO-HL60) of DHEA. During the differentiation, activity, mRNA and protein levels of G6PD were increased. DHEA increased these levels further. DHEA by itself suppressed the production of superoxide from DMSO-HL60 upon stimulation with phorbol myristate acetate (PMA). However, DMSO-HL60/DHEA stimulated with PMA in the absence of DHEA produced superoxide and 8-oxo-deoxyguanosine more than PMA-stimulated DMSO-HL60. After addition of H(2)O(2), the ratio of reduced glutathione to oxidized glutathione was lower in DMSO-HL60/DHEA than in DMSO-HL60. These findings indicate that DHEA acts both as an antioxidant and as a pro-oxidant.
- Published
- 2009
- Full Text
- View/download PDF