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In the present study, genetic screening of Sahiwal and Holstein Friesian (HF) cattle and its crosses was conducted using the PCR-RFLP technique to detect the presence of CVM and DUMPS disorders. Genomic DNA was extracted from 50 Sahiwal and 100 HF animals and its crosses using commercially available kits. Gene-specific primers were utilized for in vitro amplification of SLC35A3 and UMPS genes by PCR, followed by RFLP analysis using PstI and AvaI restriction endonucleases for CVM and DUMPS, respectively. Subsequently, the PCR products were purified and sequenced to confirm any mutations in the DNA target sequences. Notably, no affected or carrier animals for CVM and DUMPS disorders were identified among the screened animals. Thus, the gene and genotype frequency of CVM and DUMPS carriers was zero, indicating a 100% frequency of normal animals. The study underscores the importance of continued screening for these genetic disorders, involving a larger sample size across the state, and advocates for mandatory screening before utilizing animals for breeding purposes, in adherence to minimum standard protocols.

Present study includes the effect of inbreeding on expected genetic gain and estimation of regression with respect to first lactation production, reproduction and lifetime traits in Karan Fries crossbred cattle maintained at ICAR-NDRI, Karnal. Out of total, only 36.97% were found to be inbred with an average inbreeding coefficient 3.68%. Overall least squares mean for reproduction traits (days) AFC, SP, DP and CI were 1020.41 ± 5.49, 141.42± 3.86, 74.54 ± 2.04 and 421.20 ± 3.81 respectively. For production traits (kg) were 305MY, LL, LTMY, FY and SNFY were 3169.15 ± 37.87, 353.25 ± 4.18, 3686.10 ± 55.10, 266.19 ± 4.66 and 127.81 ± 2.26 respectively and lifetime traits like LTMY and stayability were 14588.47 ± 486.09 (kg) and 2444.69 ± 41.26 (days) respectively. Among reproduction traits (days), AFC increased by +3.70, DP +3.66 and CI +68.44 however SP decreased by -0.85 d and production traits (kg), 305MY decreased by -10.2 TMY -16.09, LL -1.23 d, 305FY -1.75, 305SNFY - 0.26, LTMY 202.02 and stayability -17.37 days per unit increase in the inbreeding coefficient. Although there was no inbreeding depression in any of the traits except AFC. The expected genetic gain of AFC increased two folds in IBL2 (Fx >5%) as compared to IBL1 (Fx

The present experiment was conducted at Bull Mother Experimental Farm (BMEF), College of Veterinary Science and Animal Husbandry, Anjora to study the relationship between skin thickness and milk yield of Sahiwal cows. The overall mean skin thickness of neck, dewlap, chest, abdomen, flank, hind quarter, udder and rump was 3.44±0.06, 3.49±0.06, 3.53±0.06, 4.53±0.08, 5.62±0.12, 4.07±0.07, 2.45±0.04 and 6.63±0.17 mm respectively. Analysis of variance showed that that the effect of skin thickness was statistically significant (P

There are 23 descript breeds of goat in India. However there is no recognized breed of goat registered by Chhattisgarh state hence requires systematic study for their characterization. The present study was undertaken to establish the norms of different physical characteristics, reproductive and productive performance traits and haematological profile of local goats of Chhattisgarh plains. Information was collected from Raipur, Durg, Rajnandgaon and Kanker districts of Chhattisgarh plain region. The average measurement for body weight, body length, height, ear length, face length, horn length and tail length for adult male were 31.05±0.49kg, 71.26±0.6, 74.44±0.91, 45.35±0.71, 16.14±0.21, 19.47± 0.18, 15.85±0.43 and 16.05 cm respectively. The corresponding values in adult female were 24.55±0.47 kg, 61.13±0.90, 64.74±0.73, 37.37±0.52, 15.74±0.24, 18.41±0.18, 11.72±0.57, and 14.58±0.22 cm respectively. The mean values for the reproductive performance traits i.e. age at first mating in male, age at first mating in female, age at first kidding and kidding interval were observed as 184±0.05, 216±0.04, 370±0.4 and 184±0.03 days, respectively, and average litter size 1.5. The mean values for the productive performance traits daily milk yield, total lactation milk yield and lactation length were recorded as 307.25±13.16 ml, 32.29±2.65 litres, and 105.75±2.48 days, respectively. Different haematological parameters such as haemoglobin, packed cell volume, total erythrocytes count, total leukocytes count, mean corpuscular haemoglobin, mean corpuscular volume and mean corpuscular haemoglobin concentration were considered and value estimated as 9.35±0.20 (g%), 28.9±0.66 (%), 8.45+0.17 (million/μl), 9,660±256.33 (thousand/μl), 9.1± 0.22 (pg), 27.79±0.0 (fl), and 32.39±0.27(%) respectively. Hence this study was aimed to phenotypically characterize the local goat in view of establishing a database for the breed and undertaking conservation programme for preserving rich indigenous genetic resources biodiversity.