1. Lack of salidroside impact on selected cytochromes encoding genes transcription in the liver of ethanol induced rats.
- Author
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Kujawski, Radosław, Szulc, Michał, Toboła, Maria, Graczyk, Marcin, Czora-Poczwardowska, Kamila, Baraniak, Justyna, Kania-Dobrowolska, Małgorzata, Słyńko-Krzyżostaniak, Julia, Krajewska-Patan, Anna, and Mikołajczak, Przemysław Ł.
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CYTOCHROMES ,ETHANOL ,GENETIC transcription ,POLYMERASE chain reaction ,MESSENGER RNA - Abstract
Introduction: The molecular basis of in vivo metabolism of selected representatives of phenylethanoids in the presence of ethanol has not been fully elucidated. Objective: The aim was to estimate a salidroside (Sal) metabolism in the liver tissue in rats with induced alcohol tolerance by assessing changes in the transcription of genes encoding cytochromes: CYP1A2, 2D2, 3A1, 2C23. Methods: cDNA was synthesized from total RNA isolated from rat liver samples. mRNA level changes were evaluated using real-time PCR (qRT-PCR) technique. Results: Ethanol caused a significant induction of the CYP1A2 and CYP2C23 genes transcription, and a decrease in the CYP3A1 mRNA level, predominantly without statistical significance. A statistically significant increase of the CYP1A2 mRNA level was observed in the group receiving only Sal (4.5 mg/kg b.w.; p.o.) (p<0.01). Conclusions: There was no unequivocal effect of salidroside on the transcription of investigated cytochrome genes in the liver of rats with induced alcohol tolerance. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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