1. Collagen/chitosan hybrid 3D-scaffolds as potential biomaterials for tissue engineering
- Author
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Marianelly Esquivel, Sergio Madrigal-Carballo, Daniel Esquivel-Alvarado, Hilary Urena-Saborio, and Emilia Alfaro-Viquez
- Subjects
Chemistry ,Biomedical Engineering ,Biomaterial ,Matrix (biology) ,Biodegradation ,Atomic and Molecular Physics, and Optics ,Chitosan ,Biomaterials ,chemistry.chemical_compound ,Chemical engineering ,Tissue engineering ,Cell culture ,Thermal stability ,Physical and Theoretical Chemistry ,Porosity - Abstract
Chitosan (CHT) has been reported to be biocompatible, bio-absorbable and particularly, is considered a good wound-healing accelerator. On the other hand, collagen (CGN) is one of the most widely used matrix biomaterial in tissue engineering. Highly porous CGN single 3D-scaffolds have been used to support in vitro growth of many types of tissues. We fabricated hybrid 3D-scaffold biomaterials by mixing CHT from native shrimp waste streams and CGN from tilapia aquaculture waste by-products, applying previously developed methodologies involving solvent casting and freeze-drying. CGN/CHT hybrid 3D-scaffolds were characterised according to its water uptake capacity, mechanical properties, thermal behaviour (TGA), and morphology (SEM). Hybrid 3D-scaffolds showed improved stability, greater porosity, increased thermal stability and mechanical properties, as well higher biodegradation as compared to single 3D-scaffolds. Cell culture incubation with adipose tissue-derived stem cell (ADSC) and SEM imaging showed that CGN/CHT hybrid 3D-scaffolds allowed ADSC adhering, spreading, and growing in vitro.
- Published
- 2018