1. MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells
- Author
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Noam Shomron, Dana Chuderland, Anna Tuvar, Ruth Shalgi, Mattan Levi, Ilan Tsarfaty, Salomon M. Stemmer, Irit Ben-Aharon, Hadas Grossman, Sofia Zilber, and Lihi Ninio-Many
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,actin cytoskeleton ,Motility ,Editorials: Cell Cycle Features ,miR-125a-3p ,migration ,Prostate cancer ,FYN ,microRNA ,Fyn ,Medicine ,Paxillin ,biology ,business.industry ,EMT ,Cancer ,Transfection ,live imaging ,medicine.disease ,Actin cytoskeleton ,prostate cancer ,Oncology ,Cancer research ,biology.protein ,biological phenomena, cell phenomena, and immunity ,business ,Research Paper - Abstract
Fyn kinase is implicated in prostate cancer. We illustrate the role of miR-125a-3p in cellular pathways accounted for motility and migration of prostate cancer cells, probably through its regulation on Fyn expression and Fyn-downstream proteins. Prostate cancer PC3 cells were transiently transfected with empty miR-Vec (control) or with miR-125a-3p. Overexpression of miR-125a-3p reduced migration of PC3 cells and increased apoptosis. Live cell confocal imaging indicated that overexpression of miR-125a-3p reduced the cells' track speed and length and impaired phenotype. Fyn, FAK and paxillin, displayed reduced activity following miR-125a-3p overexpression. Accordingly, actin rearrangement and cells' protrusion formation were impaired. An inverse correlation between miR-125a-3p and Gleason score was observed in human prostate cancer tissues. Our study demonstrated that miR-125a-3p may regulate migration of prostate cancer cells.
- Published
- 2014