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We—Waheed Ahmad, Andleeb Farooq, Tazeen, Maham Irfan and Nawal Naveed Abbasi— have made an attempt to explain the Retrospective review of microbial ecological processes to understand environmental biotechnology. The fields of environmental biotechnology and microbial ecology are two blossoming fields that have greatly benefited from the advancements in biology, engineering, computing and materials. Although both of the fields are traditionally varied, but the future of both the disciplines are linked to one another. Both the fields, together, provide and promise so much to help society, face and eradicate an environmental problems and challenges, sustainability, human health and security. Moreover, we have also talked about the microbial ecological processes to better understand environmental biotechnology, potential applications of these processes towards our own environment and the future perspective that where this technology is accelerating and heading towards, and what more methods and processes will be witnessed in near future to successfully eradicate and degrade the pollutants and contaminants from the environment through the interaction between microbial communities and their environment for a better, secure and sustainable ecosystem.