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This paper describes the simple and environmentally friendly production of AgNPs using Dimrit raisin, grown in Burdur, extract. To optimize the synthesis of AgNPs, a number of variables, including extract concentration, silver solution concentration, synthesis time, and synthesis temperature, were investigated. AgNPs were obtained after 173 h at 1% extract concentration and 10-1 M silver concentration. The structure of the synthesised AgNPs was investigated by Transmission electron microscopy (TEM), X-ray diffraction (XRD), UV-visible spectroscopy, and Fourier transform infrared spectroscopy (FT-IR). TEM analysis showed that the majority of the AgNPs had a spherical shape, and the average particle size was 30 nm. Antimicrobial activity was seen against Staphylococcus aureus ATTC43300, Enterococcus faecalis ATTC29212, Bacillus subtilis, Listeria monocytogenes, Klebsiella pneumoniae and Gram-negative Salmonella enterocolitis, E. coli 0157:H7 ATTC 43895, and Escherichia coli ATTC 35150. [ABSTRACT FROM AUTHOR]